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Horm Metab Res 1970; 2(6): 352-356
DOI: 10.1055/s-0028-1095053
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© Georg Thieme Verlag KG Stuttgart · New York

In Vivo Bioassay of ACTH: New Developments

M.  Chrétien , G.  Smith
  • Clinical Research Institute of Montreal, Montreal, Canada
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Publication Date:
08 January 2009 (online)

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Abstract

Using the method of corticosteroid binding protein for measuring corticosterone in rat plasma, we have measured the biological activity of ACTH after intravenous injections in hypophysectomized rats. This bioassay has sensitivity, specificity and reproducibility comparable to those using different methods for measuring blood corticosterone. Only 0.02 ml of plasma is needed. The lower limit of sensitivity is 0.2 mU of ACTH, but the accuracy is maximal between 1 and 4 mU.

Key words

ACTH - Corticosterone - Competitive Protein Binding Analysis