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Horm Metab Res 1969; 1(1): 32-35
DOI: 10.1055/s-0028-1095171
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© Georg Thieme Verlag KG Stuttgart · New York

Thin Layer Chromatography of Urinary Acid Glycosaminoglycans as Screening Procedure for Mucopolysaccharidoses[*]

W. M. Teller , A.  Ziemann
  • The Departments of Pediatrics, Universities of Heidelberg and Marburg/Lahn, Germany
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Publication History

Publication Date:
08 January 2009 (online)

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Abstract

The paper chromatographic system of SPOLTER and MARX (1960) is applied to thin layer chromatography. With a formate buffer/isopropanol relationship of 60:40 satisfactory separation of the main urinary acid glycosaminoglycans can be achieved within 5 to 6 hours. Mucopolysaccharidoses types I + II, III, V, and VI may be identified using thin layer chromatography instead of the more time consuming column chromatography.

Key words

Thin Layer Chromatography - Urinary Acid Glycosaminoglycans

1 Supported by a grant from the Deutsche Forschungsgemeinschaft, Bad Godesberg, Germany

1 Supported by a grant from the Deutsche Forschungsgemeinschaft, Bad Godesberg, Germany