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Planta Med 1979; 36(5): 30-42
DOI: 10.1055/s-0028-1097237
Research Articles

© Georg Thieme Verlag Stuttgart · New York

HPLC Trennung und quantitative Bestimmung der Ginsenoside von Panax ginseng, Panax quinquefolium und von Ginseng–Spezialitäten

1. Mitteilung1 HPLC Separation and Quantitative Determination of Ginsenosides from Panax ginseng, Panax quinquefolium and from Ginseng Drug PreparationsO. Sticher, F. Soldati2
  • Eidgenössische Technische Hochschule Zürich, Pharmazeutisches Institut, Zürich, Schweiz.
1 Erweiterte Fassung des Vortrages „Einsatz der HPLC für die Analyse der Saponine von Radix Ginseng”, 25. Jahrestagung der Gesellschaft für Arzneipflanzenforschung Zürich, 12.-16. September 1977 [I]. 2 Teil einer Dissertation: F. Soldati, ETH Zürich, Nr. 6347, 1979.
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Publication Date:
13 January 2009 (online)

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Abstract

A new method for separation and quantitative determination of ginsenosides Rg2, Rf, Rg1 and Re in Panax ginseng, Panax quinquefolium and in pharmaceutical drug preparations with HPLC was elaborated. μPorasil column using n–heptane–n–butanol–aceto–nitril–water (1000:446:132:36) as mobile phase was employed. The ginsenosides were separated in 40 min.

A reversed–phase–system with a μBondapak C18 column using methanol–water (440:560) as eluent was suitable for the separation of ginsenoside Rg1. The reversed–phase–system is adoptable for routine analysis of Ginseng extracts. The detection limit for Rgt with the spectrophotometer LC–55 (Perkin–Elmer) was at 207 nm 300 ng at a signal–to–noice ratio of 10:1.

The relative standard deviation is depending from the content of ginsenosides. For ginsenoside contents of 1 % it was circa 1 °/o.

Key Word Index

Panax ginseng - Panax quinquefolium - Ginsenosides Rg2, Rf, Rg1 - Re - Triterpene Saponines - HPLC