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Planta Med 1977; 32(6): 115-117
DOI: 10.1055/s-0028-1097568
© Georg Thieme Verlag Stuttgart · New York

TISSUE CULTURE OF STEVIA REBAUDIANA, A SWEETENING PLANT

Walter Handro, Kurt G. Hell, Gilberto B. Kerbauy
  • Plant Tissue Culture Laboratory, Dept. of Botany, USP, Sao Paulo, Brasil
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Publication Date:
13 January 2009 (online)

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Abstract

Leaf and stem expiants of Ste via rebaudiana are able to produce vigorous calluses when cultured on synthetic media containing one auxin and one cytokinin. Callus fragments may be isolated and maintained in culture during an indefinite number of transfers without losing its proliferative ability. This paper also reports the in vitro culture of small stem cuttings.