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Planta Med 1974; 25(4): 396-400
DOI: 10.1055/s-0028-1097961
© Georg Thieme Verlag Stuttgart · New York

A CHROMATOGRAPHIC METHOD OF ASSAY OF COLCHICINE ALKALOID

Fawzy T. Hussein, M. Adel A. Nasra
  • Faculty of Agriculture, Tripoli, University, Tripoli, Lybia
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Publication Date:
14 January 2009 (online)

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Abstract

A Chromatographie method of assay of colchicine is proposed. The liquid extract of colchicine is concentrated to a known volume, triturated with alumina (B.D.H.) to a fine powder, dried on a water bath and transferred to a column of silica gel (B.D.H.) and aluminum oxide (B.D.H.).

The alkaloid colchicine is eluted with pure acetone.

The eluent is evaporated completely and the residue is dried at 80 °C for half-an-hour and determined gravimetrically.