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DOI: 10.1055/s-0028-1128145
© Georg Thieme Verlag KG Stuttgart · New York
Effect of Sibutramine on Na+, K+ ATPase Activity and Tryptophan Levels on Male and Female Rat Brain
Publikationsverlauf
received 08.08.2008
accepted 15.12.2008
Publikationsdatum:
04. Februar 2009 (online)
![](https://www.thieme-connect.de/media/hmr/200905/lookinside/thumbnails/10.1055-s-0028-1128145-1.jpg)
Abstract
Some drugs that are clinically used in weight control, like sibutramine, act on the serotonergic metabolism, but its relation with free radical (FR) production in the CNS is still unknown. The aim of the work was to evaluate the effect of sibutramine on FR production. Female and male Wistar rats (250 g weight) were used; the animals received sibutramine (10 mg/kg each 36 hours) intraperitoneally during 15 days. At the end of the study, the rats were sacrificed and their brains used to measure lipid peroxida-tion (TBARS), Na+, K+ATPase activity, reduced glutathione (GSH), and tryptophan (TRP) levels, by means of validated methods. The activity of Na+, K+ATPase and total ATPase was increased in males and decreased in females. GSH concentration was increased and the levels of TBARS decreased by an effect related to sibutramine in the female group. Sibutramine decreased TRP concentration in the female group, but increased it in the male one, with respect to the control group. Our results suggest that sibutramine produce an antioxidant effect stimulated by the endogenously produced tryptophan and it protects the fluidity of plasma membrane in rat brain.
Key words
obesity - free radicals - ATPase - tryptophan - brain
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Correspondence
H. J. Olguín
Laboratorio de Farmacología
Instituto Nacional de Pediatría
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