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Planta Med 2009; 75(6): 629-631
DOI: 10.1055/s-0029-1185401
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Pharmacokinetic Analysis of Glaucocalyxin A in Rat Plasma by Liquid-Liquid Extraction and HPLC

Luye Cao1 , Jin Sun1 , Yun Shen Li2 , Zijun Chen3 , Yikui Li3
  • 1Food and Pharmaceutics Vocational College of Guangdong, Guangzhou, P. R. China
  • 2Laboratory of Phytochemistry, Shanghai Institute of Pharmaceutical Research, Shanghai, P. R. China
  • 3Department of Chinese Traditional Herb, Traditional Chinese Medicine of Shanghai, Shanghai, P. R. China
Further Information

Publication History

received October 29, 2008 revised December 20, 2008

accepted December 25, 2008

Publication Date:
04 March 2009 (online)

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Abstract

A validated HPLC method is described for determination of glaucocalyxin A in rat plasma using liquid-liquid extraction and UV absorbance detection. The extraction recovery of glaucocalyxin A ranged from 81.72 % to 79.25 %, the linear range was 0.2–10 µg/mL, and r was 0.9984. A pharmacokinetic analysis of glaucocalyxin A after intravenous administration in rats revealed that glaucocalyxin A followed a two-compartment open model. The values of t1/2α and t1/2β were 4.327 min and 28.56 min, respectively, and the area under the plasma concentration-time curve (AUC) was 222.744 µg · min · mL−1. The method developed was simple, rapid and specific, and an accurate way of investigating the pharmacokinetics of a diterpenoid in plasma.

Key words

pharmacokinetics - glaucocalyxin A - HPLC

References

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Dr. Luye Cao

Department of Traditional Chinese Medicine
Food and Drug Vocational College of Guangdong

Longdong Northern Street, tianhe area

Guangzhou 510520

People's Republic of China

Phone: + 86 20 28 85 49 35

Fax: + 86 20 28 85 49 10

Email: yncly001@yahoo.com.cn