Abstract
The accurate identification of medicinal plants is becoming increasingly important
due to reported concerns about purity, quality and safety. The previously developed
prototype subtracted diversity array (SDA) had been validated for the ability to distinguish
clade-level targets in a phylogenetically accurate manner. This study represents the
rigorous investigation of the SDA for genotyping capabilities, including the genotyping
of plant species not included during the construction of the SDA, as well as to lower
classification levels including family and species. The results show that the SDA,
in its current form, has the ability to accurately genotype species not included during
SDA development to clade level. Additionally, for those species that were included
during SDA development, genotyping is successful to the family level, and to the species
level with minor exceptions. Twenty polymorphic SDA features were sequenced in a first
attempt to characterize the polymorphic DNA between species, which showed that transposon-like
sequences may be valuable as polymorphic features to differentiate angiosperm families
and species. Future refinements of the SDA to allow more sensitive genotyping are
discussed with the overall goal of accurate medicinal plant identification in mind.
Key words
genotyping - microarray - diversity - phylogenetics - angiosperms
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A/Prof Eddie Pang
School of Applied Sciences, Biotechnology and Environmental Biology
RMIT University
Plenty Road
Bundoora
VIC, 3083
Australia
Telefon: + 6 13 99 25 71 37
Fax: + 6 13 99 25 71 10
eMail: eddie.pang@rmit.edu.au