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DOI: 10.1055/s-0029-1185521
© Georg Thieme Verlag KG Stuttgart · New York
Hydrophilic Ester-Bearing Chlorogenic Acid Binds to a Novel Domain to Inhibit Xanthine Oxidase
Publikationsverlauf
received Oct. 28, 2008
revised February 13, 2009
accepted February 25, 2009
Publikationsdatum:
27. März 2009 (online)
![](https://www.thieme-connect.de/media/plantamedica/200911/lookinside/thumbnails/10.1055-s-0029-1185521-1.jpg)
Abstract
Caffeic acid is a xanthine oxidase (XO) inhibitor that binds to the molybdopterin region of its active site. Caffeic acid phenethyl ester (CAPE) has higher hydrophobicity and exhibits stronger inhibition potency toward XO. Chlorogenic acid is a quinyl ester of caffeic acid that has increased hydrophilicity and also shows stronger XO inhibitory activity compared with caffeic acid. Caffeic acid and CAPE showed competitive inhibition against XO, whereas chlorogenic acid displayed mixed-type inhibition, implying that it binds to sites other than the active site. Structure-based molecular modeling was performed to account for the different binding characteristics of the hydrophobic and hydrophilic esters of caffeic acid. Chlorogenic acid showed weak binding to the molybdopterin region of XO, while it more strongly bound the flavin adenine dinucleotide region than it did the molybdopterin region. These results provide the basis for interactions of caffeic acid analogues with XO via various binding domains.
Key words
caffeic acid - chlorogenic acid - FAD - molecular modeling - molybdopterin - xanthine oxidase
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Dr. Chun-Mao Lin
College of Medicine,
Taipei Medical University
250 Wu-Hsing Street
Taipei 110
Taiwan
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