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DOI: 10.1055/s-0029-1185540
© Georg Thieme Verlag KG Stuttgart · New York
Effects of 2,3,4′,5-Tetrahydroxystilbene 2-O-β-D-Glucoside on Vascular Endothelial Dysfunction in Atherogenic-Diet Rats
Publikationsverlauf
received Nov. 25, 2008
revised February 19, 2009
accepted February 20, 2009
Publikationsdatum:
06. April 2009 (online)
![](https://www.thieme-connect.de/media/plantamedica/200911/lookinside/thumbnails/10.1055-s-0029-1185540-1.jpg)
Abstract
2,3,4′,5-Tetrahydroxystilbene 2-O-β-D-glucoside (TSG), an active component extracted from Polygonum multiflorum, has been found to have an anti-atherosclerotic effect. The aim of this study was to investigate whether the TSG could prevent the development of atherosclerosis through influencing endothelial function in atherogenic-diet rats and to explore the possible mechanisms. Vascular endothelial dysfunction was assessed using isolated aortic ring preparation, transmission electron microscopy of the aorta, and levels of nitrate/nitrite (NOx) in serum and aorta. Endothelial nitric oxide (NO) synthase (eNOS) and inducible NO synthase (iNOS) mRNA and protein expression were also measured. After 12 weeks treatment, TSG improved acetylcholine-induced endothelium-dependent relaxation, prevented intimal remodeling, inhibited the decreased NOx content in serum and aorta in atherogenic-diet rats. Furthermore, the observed decreased eNOS mRNA and protein expression and increased iNOS mRNA and protein expression in atherogenic-diet rats were attenuated by TSG treatment. These results suggest that TSG could restore vascular endothelial function, which may be related to its ability to prevent changes of eNOS and iNOS expression, leading to preservation of NO bioactivity.
Key words
2,3,4′,5‐tetrahydroxystilbene 2‐O‐β‐D‐glucoside - atherosclerosis - endothelial dysfunction - nitric oxide - nitric oxide synthase
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Wei-Zhong Zhu
Department of Pharmacology
School of Medicine
Nantong University
19 Qi Xiu Road
Nantong 226001
People's Republic of China
Telefon: + 86 5 13 85 05 17 28
Fax: + 86 5 13 85 05 18 58
eMail: zhuwz@ntu.edu.cn