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Planta Med 2009; 75(12): 1331-1335
DOI: 10.1055/s-0029-1185546
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

HIV-1-Inhibiting Activity of the Essential Oil of Ridolfia segetum and Oenanthe crocata

Carlo Bicchi1 , Patrizia Rubiolo1 , Mauro Ballero2 , Cinzia Sanna2 , Maura Matteodo1 , Francesca Esposito3 , Luca Zinzula3 , Enzo Tramontano3
  • 1Dipartimento di Scienza e Tecnologia del Farmaco University of Torino, Torino, Italy
  • 2Co.S.Me.Se., Department of Botanical Sciences, University of Cagliari, Cagliari, Italy
  • 3Department of Biomedical Science and Technology, University of Cagliari, Cagliari, Italy
Weitere Informationen

Publikationsverlauf

received October 23, 2008 revised February 27, 2009

accepted March 4, 2009

Publikationsdatum:
03. April 2009 (online)

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Abstract

The essential oils of Ridolfia segetum (L.) Moris and Oenanthe crocata L. (Apiaceae), collected in Sardinia (Italy), have been assayed for two enzyme-associated activities of the HIV-1 reverse transcriptase (RT): RNA-dependent DNA polymerase (RDDP) activity and ribonuclease H (RNase H) activity. In biochemical assays, the essential oils inhibited HIV-1 RT RDDP activity in a dose-dependent manner, while they were inactive towards RNase H activity. Furthermore, the oils were cytotoxic towards K562 cell replication. GC‐MS analysis of the essential oils obtained by steam distillation of the aerial parts showed that the main components of R. segetum were alpha-phellandrene, alpha-terpinolene, beta-phellandrene, and dillapiol and those of O. crocata were sabinene, trans-beta-ocimene, cis-beta-ocimene, and beta-pinene.

Key words

Ridolfia segetum - Oenanthe crocata - Apiaceae - essential oil - HIV‐1 reverse transcriptase inhibition - K562 cell - GC‐MS analysis

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Prof. Mauro Ballero

Co.S.Me.Se.
Department of Botanical Sciences
University of Cagliari

viale Sant'Ignazio 13

09123 Cagliari

Italy

Telefon: + 39 07 06 75 35 06

Fax: + 39 07 06 75 35 03

eMail: ballero@unica.it