Abstract
Saponinum album (SAP) is a complex mixture of triterpene saponins from Gypsophila paniculata L. Although most of the saponins from SAP are characterized, the separation of pure saponins remains time consuming and costly, involving different chromatographic techniques. Recently it was shown that SAP drastically enhanced the cytotoxicity of a chimeric toxin consisting of the N-glycosidase saporin and human epidermal growth factor (Sap-EGF) in cell culture experiments. In view of a potential therapeutic use of the coadministration of SAP and Sap-EGF in tumor therapy, an economic and time-saving method for the isolation of pure saponins from the crude SAP mixture in high amounts is required. In this study we isolated a single saponin by a simple chromatographic method. The isolated saponin was characterized by mass spectrometry and was shown to enhance the cytotoxicity of Sap-EGF on HER14 cells.
Key words
Gypsophila paniculata L.
- Caryophyllaceae - saponinum album - targeted tumor therapy
References
-
1
Frechet D, Christ B, du Sorbier B M, Fischer H, Vuilhorgne M.
Four triterpenoid saponins from dried roots of Gypsophila species.
Phytochemistry.
1991;
30
927-931
-
2
Delay C, Gavin J A, Aumelas A, Bonnet P A, Roumestand C.
Isolation and structure elucidation of a highly haemolytic saponin from the Merck saponin extract using gradient-enhanced NMR techniques.
Carbohydr Res.
1997;
302
67-78
-
3
Kim Y C, Higuchi R, Komori T.
Application of hydrothermolysis to the studies on the constituents of the Merck saponin.
Liebigs Ann Chem.
1992;
9
941-946
-
4
Elgamal A HM, Soliman M SH, Karawya M S, Mikhova B, Duddeck H.
Isolation of triterpene saponins from Gypsophila capillaries.
Phytochemistry.
1995;
38
1481-1485
-
5
Bai H, Zhong Y, Xie Y Y, Wang Y S, Liu L, Zhou L, Wang J, Mu Y L, Zou C X.
A major triterpenoid saponin from Gypsophila oldhamiana.
Chem Biodivers.
2007;
4
955-960
-
6
Heisler I, Sutherland M, Bachran C, Hebestreit P, Schnittger A, Melzig M F, Fuchs H.
Combined application of saponin and chimeric toxins drastically enhances the targeted cytotoxicity on tumor cells.
J Control Release.
2005;
106
123-137
-
7 Bachran C, Melzig M F, Heisler I, Hebesreit P, Fuchs H, Sutherland M. Composition comprising a pharmacologically active agent coupled to a target cell specific component, and a saponin. United States Patent Application 20080064762
Univ.-Prof. Dr. M. F. Melzig
Institute of Pharmacy
Free University Berlin
14195 Berlin
Germany
Telefon: + 49 30 83 85 14 51
Fax: + 49 30 83 85 14 61
eMail: melzig@zedat.fu-berlin.de
