Planta Med 2009; 75(14): 1494-1498
DOI: 10.1055/s-0029-1185798
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Suppression of the Antigen-Stimulated RBL-2H3 Mast Cell Activation by Artekeiskeanol A

JangJa Hong1 , Hirokazu Sasaki2 , Noriyasu Hirasawa2 , Kenji Ishihara2 , Jong Hwan Kwak3 , OkPyo Zee3 , Francis J. Schmitz4 , Toshio Seyama1 , Kazuo Ohuchi1
  • 1Laboratory of Life Sciences, Faculty of Pharmacy, Yasuda Women's University, Hiroshima, Japan
  • 2Laboratory of Pathophysiological Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan
  • 3Laboratory of Pharmacognosy, Graduate School of Pharmacy, Sungkyunkwan University, Suwon, Korea
  • 4Department of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma, USA
Further Information

Publication History

received February 15, 2009 revised May 1, 2009

accepted May 6, 2009

Publication Date:
01 July 2009 (online)

Abstract

Effects of artekeiskeanol A, a newly isolated coumarin derivative from Artemisa keiskeana Miq. (Compositae), the extract of which is used for treatment of rheumatoid arthritis as a folk medicine, on the antigen-induced activation of RBL-2H3 cells were examined. RBL-2H3 cells were sensitized with dinitrophenol (DNP)-specific IgE, and then stimulated with the antigen DNP-conjugated human serum albumin (DNP‐HSA). Artekeiskeanol A at 10 to 100 µM inhibited the antigen-induced degranulation in a concentration-dependent manner, the IC50 value being 38.0 ± 0.2 µM. Degranulation induced by thapsigargin or A23187 also was inhibited by artekeiskeanol A at 10 to 100 µM. The antigen-induced increase in the levels of mRNA for tumor necrosis factor (TNF)-α and interleukin (IL)-13 and phosphorylations of Akt, p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK) and p44/42 MAPK were also suppressed by artekeiskeanol A. Our findings suggested that the effectiveness of the extract of A. keiskeana might partly be due to the inhibition of mast cell activation by artekeiskeanol A.

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Ph.D. JangJa Hong

Laboratory of Life Sciences
Faculty of Pharmacy
Yasuda Women's University

6–13–1 Yasuhigashi

Asaminami-ku

731–0153 Hiroshima

Japan

Phone: + 81 8 28 78 94 51

Fax: + 81 8 28 78 28 96

Email: hong@yasuda-u.ac.jp