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DOI: 10.1055/s-0029-1185806
© Georg Thieme Verlag KG Stuttgart · New York
Protective Effects of Echinacoside, One of the Phenylethanoid Glycosides, on H2O2-Induced Cytotoxicity in PC12 Cells
Publikationsverlauf
received January 21, 2009
revised May 7, 2009
accepted May 14, 2009
Publikationsdatum:
22. Juni 2009 (online)
![](https://www.thieme-connect.de/media/plantamedica/200914/lookinside/thumbnails/10.1055-s-0029-1185806-1.jpg)
Abstract
We have investigated the protective effects of echinacoside (ECH), one of the phenylethanoid glycosides, on H2O2-induced cytotoxicity in the rat pheochromocytoma cell line (PC12 cells). Our data show that application of ECH to H2O2-injured PC12 cells (HIPCs) increased cell viability and decreased the apoptotic ratio. Flow cytometry (FCM) and laser scanning confocal microscopy (LSCM) analysis suggested that ECH exerted its inhibitory effects on the formation of reactive oxygen species (ROS) and the accumulation of intracellular free Ca2+ ([Ca2+]i). In addition, ECH elevated the mitochondrial membrane potential (MMP) in HIPCs. Furthermore, Western blot analysis revealed that ECH prevented an H2O2-induced increase of the Bax/Bcl-2 ratio by down-regulating Bax protein expression and upregulating Bcl-2 protein expression. In summary, ECH showed significant neuroprotective effects on HIPCs through the mitochondrial apoptotic pathway, and could be a potential candidate for intervention in neurodegenerative diseases such as Alzheimer's and Parkinson's disease.
Key words
echinacoside (ECH) - apoptosis - ROS - MMP - [Ca2+]i - Bax/Bcl‐2
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Professor Xiaoxiang Zheng
Department of Biomedical Engineering
Key Laboratory of Biomedical Engineering
Ministry of Education
Zhejiang University
Zheda Road 38
310027 Hang Zhou
People's Republic of China
Telefon: + 86 5 71 87 95 38 60
Fax: + 86 5 71 87 95 16 76
eMail: zxx@mail.bme.zju.edu.cn