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DOI: 10.1055/s-0029-1185903
© Georg Thieme Verlag KG Stuttgart · New York
Neuroprotective Effect of Defatted Sesame Seeds Extract against in vitro and in vivo Ischemic Neuronal Damage
Publication History
received Dec. 18, 2008
revised June 5, 2009
accepted June 10, 2009
Publication Date:
13 July 2009 (online)
![](https://www.thieme-connect.de/media/plantamedica/201001/lookinside/thumbnails/10.1055-s-0029-1185903-1.jpg)
Abstract
Sesame (Sesamum indicum L.) is an important oilseed crop that possesses a wide spectrum of pharmacological activities. Many studies have been conducted to investigate its health-promoting effects. Compared to other plant oils, sesame seed oil is highly stable to oxidation and has been demonstrated to have protective effects against ischemia-reperfusion injury in the rat brain. However; the effects of defatted sesame seeds extract (DSE) have not been studied yet. The purpose of this study was to evaluate the protective effect of DSE against ischemia models. For in vitro ischemia, oxygen-glucose deprivation followed by reoxygenation (OGD‐R, 4 h OGD followed by 24 h reoxygenation) in HT22 cells was used to investigate the protective effects on cell death and the inhibitory effects on lipid peroxidation. For in vivo ischemia, the middle cerebral artery occlusion (MCAo, 2 h of MCAo followed by 22 h of reperfusion) rat model was used. Twenty-two h after occlusion the rats were assessed for neurobehavioral deficit and infarct volume. DSE (0.1–10 µg/mL) significantly reduced the cell death and inhibited lipid peroxidation induced by OGD‐R. DSE (30, 100 and 300 mg/kg, p. o.) given twice at 0 h and 2 h after onset of ischemia reduced brain infarct volume dose-dependently and improved sensory-motor function. The therapeutic time window of DSE (300 mg/kg, p. o.) was 2 h after MCAo in rats. In conclusion, our results show that DSE may be effective in ischemia models by an antioxidative mechanism.
Key words
Sesamum indicum L. - Pedaliaceae - defatted sesame seeds extract - neuroprotection - cerebral ischemia
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Prof. Youngmin Bu
Department of Herbal Pharmacology
College of Oriental Medicine
Kyung Hee University
Seoul 130-701
Korea
Phone: + 82 29 61 94 62
Fax: + 82 29 64 03 25
Email: ymbu@khu.ac.kr