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DOI: 10.1055/s-0029-1186055
© Georg Thieme Verlag KG Stuttgart · New York
Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice
Publication History
received February 19, 2009
revised June 15, 2009
accepted July 24, 2009
Publication Date:
25 August 2009 (online)
Abstract
Ganoderma lucidum is a well-known traditional Chinese medicine and often used for improving quality of life. Ganoderma lucidum polysaccharide (GLP) is the main active component in the fungus. Recent researches have shown that oral administration of GLP could produce immunomodulation and antitumor activity. With a high molecular weight, GLP is not easy to be absorbed, and the pathway via enteric mucosal immune response might be important for the immunomodulation of GLP. To investigate the potential mechanism, Kunming mice, weighing 20 ± 2 g, were used and their peripheral blood mononuclear cells (PBMC), intraepithelial lymphocytes (IEL) and Peyer's patches lymphocytes (PPL) were separated, and incubated with GLP at different dosages (250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.25 µg/mL). MTT, ELISA and RT‐PCR were used to detect the effects of GLP on the proliferation of the lymphocytes, and expression of IL-10, IL-2 and TNF-α. The results showed that GLP could stimulate the proliferation of PBMC and enteric mucosal lymphocytes, and promote the production of IL-2 and IL-10. The RT‐PCR results also showed a higher expression of TNF-α and IL-10 mRNA in the lymphocytes. The higher expression of IL-2, IL-10 and TNF-α from the lymphocytes was observed at GLP concentrations of 62.5 µg/mL and 125 µg/mL. In conclusion, enteric mucosal immune responses could be one of the important pathways for the immunomodulation activity of GLP.
Key words
mucosal immune response - cytokines - Ganoderma lucidum polysaccharide - Ganoderma lucidum - Polyporaceae
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PhD, MD Aiping Lu
Institute of Basic Research in Clinical Medicine
China Academy of Chinese Medical Sciences
No. 16, Nanxiaojie, Dongzhimennei
Beijing 100700
People's Republic of China
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