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Int J Sports Med 2009; 30(7): 550-554
DOI: 10.1055/s-0029-1202342
Clinical Sciences

© Georg Thieme Verlag KG Stuttgart · New York

MMP-2 is not Altered By Stretching in Skeletal Muscle

S. M. Peviani 1 , A. R. S. Gomes 2 , H. S. Selistre de Araujo 3 , T. F. Salvini 1
  • 1Department of Physical Therapy, Federal University of São Carlos, São Carlos, Brazil
  • 2Department of Physical Therapy, Federal University of Paraná, Matinhos, Brazil
  • 3Department of Physiology, Federal University of São Carlos, São Carlos, Brazil
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Publication History

accepted after revision January 10, 2009

Publication Date:
19 March 2009 (online)

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Abstract

Considering that short bouts of stretching, as recommended in rehabilitation and sports activities, induce skeletal muscle and connective tissue adaptation, the hypothesis of this study was that MMP-2 activity is regulated by muscle stretch. The level of MMP-2 activity was, thereby, assessed after stretching in rat soleus muscle. Animals received a single session of stretching (10 stretches lasting 1 min each with 30 s of rest in between) and were evaluated immediately and after 8, 24, 48, 72 and 168 h. To evaluate the effect of repetitive sessions of stretching, three groups of animals were evaluated – one group after 2 sessions, another after 3, and a third after 7. MMP-2 activity was evaluated by zymography and MMP-2 mRNA was assessed by real-time polymerase chain reaction. None of the groups presented MMP-9 activity. MMP-2 activity and mRNA expression did not change after either single or repetitive sessions of stretching. In conclusion, the results of this study indicate that MMP-2 is not involved in the muscle stretch-induced remodeling.

Key words

stretching - MMP-2 - sports activities - rehabilitation - muscle adaptation - MMP-9

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Correspondence

S. M. Peviani

Department of Physical Therapy

Federal University of São Carlos

Washington Luis

km 235

13565-905 São Carlos

Brazil

Phone: +55/16/3351 83 45

Fax: +55/16/3351 20 81

Email: sabrinapeviani@gmail.com