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Exp Clin Endocrinol Diabetes 1984; 83(1): 101-109
DOI: 10.1055/s-0029-1210317
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Does Insulin Down-Regulate Its Own Receptor on Erythrocytes in Vitro?

B. Schulz, L. Doberne, H. Keilacker, G. M. Reaven
  • Clinical Research Center (Director: G. M. Reaven, M. D.) Stanford University School of Medicine, Stanford, California/USA, Central Institute of Diabetes „Gerhardt Katsch“ (Director: OMR Prof. Dr. sc. med. H. Bibergeil), Karlsburg/GDR
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1982

Publikationsdatum:
17. Juli 2009 (online)

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Summary

The purpose of this study was to examine the in vitro effect of insulin on its own receptors on erythrocytes.

Whole blood from 6 fasting normal and 6 diabetic (type II) subjects was incubated without or with exogenous insulin for 5 hours. The insulin binding to erythrocytes was then evaluated.

At a tracer concentration, the percent 125I-insulin specifically bound amounted to 8.6 ± 0.59% and 8.1 ± 0.41% in fasting normal subjects and type II-diabetics, respectively.

In contrast to in vivo studies, supraphysiologic insulin concentrations in the incubation medium did not alter significantly the insulin binding process. Thus, in vitro we could not demonstrate a down regulation of insulin receptor binding.

It is likely that factors other than insulin are involved in the short-term regulation of insulin receptor affinity in man.

Key words

Insulin receptors - erythrocytes - affinity