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Exp Clin Endocrinol Diabetes 1985; 86(4): 61-68
DOI: 10.1055/s-0029-1210473
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

The Effect of Potassium, Calcium and Magnesium Concentration on Insulin and Glucagon Secretion of the Perfused Dog Pancreas

E. Panzig, W. Besch, K.-D. Rosenbaum, W. Tietz, S. Kiene, E. Wolf, W. Paul
  • Department of Surgery (Director: MR Prof. Dr. sc. med. S. Kiene) and Central Laboratory (Head: Prof. Dr. sc. med. W. Haude) of the Ernst-Moritz-Arndt-University Medical School, Greifswald, Central Institute of Diabetes “Gerhard Katsch” (Director: OMR Prof. Dr. sc. med. H. Bibergeil), Karlsburg/GDR
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Publication History

1984

Publication Date:
16 July 2009 (online)

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Summary

The effect of different potassium, calcium and magnesium concentrations in the perfusate on the hormone secretion of the isolated dog pancreas was investigated. A potassium concentration above 15 mMol/l shortly stimulates the insulin and glucagon secretion. Potassium ions (≥15 mMol/l) completely inhibit the early phase of glucose-induced insulin release. At a low Ca2+- level (0.25 mMol/l) the glucose-stimulated insulin secretion is reduced to basal values. On the other hand, the glucagon release is stimulated under these conditions. An increase of magnesium ions from 1.0 mMol/l to 2.5— 7.5 mMol/l strikingly inhibits insulin and glucagon release by approximately 50%, which is compensated for insulin by increasing the Ca2+- content of the medium.

Perfusates for normothermic pancreas perfusion should contain electrolyte concentrations within the physiological range.

Key words

Pancreas - Perfusion - Perfusate - Insulin - Glucagon