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Exp Clin Endocrinol Diabetes 1985; 86(5): 146-150
DOI: 10.1055/s-0029-1210480
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Measurement of Plasma Canine C-Peptide by Radioimmunoassay

W. Besch, K.-P. Woltanski, U. Fischer, K.-D. Kohnert, M. Ziegler
  • Central Institute of Diabetes “Gerhardt Katsch” (Director: OMR Prof. Dr. sc. med. H. Bibergeil), Karlsburg/GDR
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Publication History

1984

Publication Date:
16 July 2009 (online)

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Summary

A sensitive radioimmunoassay (RIA) for canine C-peptide (CCP) was established using synthetic CCP, a specific antiserum, and rabbit anti-guinea pig serum. Radioiodination was performed according to a modified chloramine-T method. Tracer preparations were used for long as 6 weeks after iodination. The standard curve ranges from 0.028 to 3.0 nmol/1. The intra-assay coefficient of variation (CV) was 3–5% and the inter-assay CV was 6–9% in the optimal range between 0.3 and 0.8 nmol/1. The average recovery of CCP added to plasma samples was 100.6% (n = 9). Canine insulin, porcine proinsulin, bovine proinsulin, and human C-peptide exhibited no cross-reactivity. The mean fasting plasma CCP concentration was 0.089 ± 0.021 nmol/1 in normal dogs and –0.005 ± 0.007 nmol/1 (mean ± SEM) in diabetic dogs, respectively.

Key words

Canine C-peptide - Radioimmunoassay - Diabetic dog