Human Pancreatic Extract and Rat Pancreatic Islet Homogenate-Coated Chicken Erythrocytes as Targets of Lymphocyte-Mediated Cytotoxicity in Type I Diabetic Patients

Mária Horváth; D. Schröder; Mária Varsányi; I. Balázsi

doi:10.1055/s-0029-1210849

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Exp Clin Endocrinol Diabetes 1989; 93(2/03): 151-156
DOI: 10.1055/s-0029-1210849

Original

Human Pancreatic Extract and Rat Pancreatic Islet Homogenate-Coated Chicken Erythrocytes as Targets of Lymphocyte-Mediated Cytotoxicity in Type I Diabetic Patients

Mária Horváth, D. Schröder¹ , Mária Varsányi, I. Balázsi

Third Department of Medicine, Semmelweis Medical University, Budapest, Hungary
¹Central Institute of Diabetes „Gerhardt Katsch“, Karlsburg, GDR

Further Information

Publication History

1988

Publication Date:
16 July 2009 (online)

Also available at

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Summary

In this study ⁵¹Cr-labeled chicken erythrocytes coated with human pancreatic extract or rat pancreatic islet homogenate served as target cells for the detection of lymphocyte-mediated cytotoxicity in type I-diabetics.

T-lymphocytes from type I-diabetic patients showed a significant cytotoxic capacity against target cells coated with human antigens as well as rat pancreatic islet homogenate. The frequency and intensity of cytotoxicity proved to be similar against the two types of targets. Since rat pancreatic islets are easily available and contain more characteristic beta-cell antigens than human pancreas extract, the homogenate of rat islets of Langerhans is more suitable than human pancreatic extract for studying the lymphocyte-mediated cytotoxicity in type I-diabetic patients.

Key Words

Human pancreatic extract - Rat pancreatic islets homogenate - Lymphocyte-mediated cytotoxicity - T-lymphocytes - Type I-diabetes mellitus