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DOI: 10.1055/s-0029-1210852
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
The Effects of Sera Obtained from Children with Insulin-Dependent Diabetes Mellitus on Cultivated Islet Cells: Cytotoxicity and Insulin Release
Publikationsverlauf
1988
Publikationsdatum:
16. Juli 2009 (online)
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Summary
Sera were obtained from 24 patients with newly-diagnosed insulin-dependent diabetes mellitus (IDDM) and 14 children with a high risk of diabetes. The influence of the decomplementated sera on basal and stimulated insulin secretion was studied in a mixed culture of newborn rat islet cells. In addition, complement-dependent antibody-mediated cytotoxicity (C'AMC) was measured by 51Cr-release from pre-labelled islet cells.
Incubation of the islet cells with sera from ten IDDM patients did not affect the basal insulin release. Sera from other children with IDDM (n = 14) either significantly increased (n = 7) or inhibited (n = 7) basal IRI secretion was compared with the sera of control donors. Nearly half of the sera from the high-risk children was found to be insulin-stimulating. Preincubation of islet cells with sera from IDDM children caused a significant decrease of insulin response to 16.5 mM glucose plus 5 mM theophylline (P < 0.001). Sera from the high-risk children did not influence the response of pancreatic cells to secretagogues. C'AMC was found in 45% of the patients with IDDM and in 33% of the high-risk children, however, there was no correlation between C'AMC and serum effect upon basal insulin secretion.
These results suggest the presence of B-cytotropic factors in serum from children with IDDM or with a risk of diabetes. Opposite effects of different sera on insulin secretion may reflect the variety of pathogenetic mechanisms involved in islet cell destruction.
Key Words
Insulin secretion - Islet cells - Complement-dependent antibody-mediated cytotoxicity - Type I Diabetes Mellitus