Monoclonal Islet Cell Surface Antibody Dependent Cellular Cytotoxicity Mediated by Rat Splenocytes with the RIN Cell Line as Target

I. Hulinsky; Brigitte Ziegler; E. Cabrera; M. Schlosser; Sabine Witt; M. Ziegler

doi:10.1055/s-0029-1210854

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Exp Clin Endocrinol Diabetes 1989; 93(2/03): 181-186
DOI: 10.1055/s-0029-1210854

Original

Monoclonal Islet Cell Surface Antibody Dependent Cellular Cytotoxicity Mediated by Rat Splenocytes with the RIN Cell Line as Target

I. Hulinsky, Brigitte Ziegler, E. Cabrera¹ , M. Schlosser, Sabine Witt, M. Ziegler^*

¹Central Institute of Diabetes “Gerhardt Katsch”, Karlsburg, GDR and National Institute of Endocrinology, Havana, Cuba

Further Information

Publication History

1988

Publication Date:
16 July 2009 (online)

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Summary

A new method is described for testing monoclonal islet cell Surface antibodies for their ability to mediate cellular immune effector mechanisms (ADCC). For the first time an IgM mediated cellular cytotoxicity is demonstrated. By using the RIN cell line as target the method was reproducible and easy to perform. The significance of such definition of monoclonal ICSA's to experimental diabetes research is discussed.

Key words

Monoclonal ICSA - Rat insulinoma cell line - ADCC