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DOI: 10.1055/s-0029-1210870
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
Radioreceptor Assay far Detergent-Solubilized Rat Insulinoma Growth Hormone Receptors
Publikationsverlauf
1988
Publikationsdatum:
16. Juli 2009 (online)
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Summary
Growth hormone receptors from a rat insulinoma cell line, BIN-5AH were solubilized in the non-ionic detergent Triton X-100. A radioreceptor assay based on polyethylene glycol precipitation of the growth hormone: receptor complex showed time-dependent and Saturable hormone binding. The affinity in detergent solution for biosynthetic human growth hormone of approx. 6 ng/ ml was found similar to that of intact KIN cells. The solubilization and receptor assay conditions described are useful for further characterization and purification of RIN cell growth hormone receptors, which might provide an initial insight into the molecular mechanism of the growth hormone effects on islet β-cells.
Key Words
Growth hormone receptor - Insulinoma cell line - Binding characteristics - Pancreatic B-cell growth