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DOI: 10.1055/s-0029-1210872
© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York
The Effects of Glibenclamide and its Non-Sulfonylurea Analogue HB 699 on the Sodium Content of Rat Pancreatic Islets
Publication History
1988
Publication Date:
16 July 2009 (online)
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Summary
Sodium contents were determined in rat pancreatic islets using integrating flame photometry. Whereas the sodium content decreased in the presence of glucose, it increased when 0.1—100 μmol/1 glibenclamide was added to a medium containing 3 mmol/1 glucose. The complexity of the glibenclamide action became evident with its reversal after removal of extracellular Ca2+ and the observation that the sulfonylurea counteracted the increase of sodium obtained after removal of K+. The effects of glibenclamide were mimicked by 1 mmol/1 of its non-sulfonylurea analogue HB 699 with the exception that the latter compound being without suppressive action on the sodium content in medium deprived of Ca2+- Also exposure to 1 mmol/1 sulfadiazine resulted in a Ca2+-dependent increase of sodium. The results suggest a role for sodium in amplifying the secretory response to the increased entry of Ca2+ obtained with the depolarisation of the β-cells with glibenclamide or HB 699.
Key Words
Pancreatic Islet - Rat - Sodium Content - Flame photometry - Calcium