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Exp Clin Endocrinol Diabetes 1990; 95(1): 148-156
DOI: 10.1055/s-0029-1210947
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Autologous Mixed Lymphocyte Reaction in Newly Diagnosed Type-1 Diabetes*

S. Knospe, Erika Köhler, Ilona Rjasanowski, Karin Titze
  • Central Institute of Diabetes “Gerhardt Katsch” (Director: OMR Prof. Dr. sc. med. H. Bibergeil), Karlsburg/GDR
* Dedicated to Professor H. Bibergeil on the occasion of his 65th birthday
Further Information

Publication History

1989

Publication Date:
16 July 2009 (online)

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Summary

The autologous mixed lymphocyte reaction (AMLR) represents activation, proliferation and differentiation of T cells in response to signals from autologous non-T cells. Deteriorations in AMLR have been reported in many autoimmune diseases and in diseases with a derangement in T cell regulatory function. We have studied AMLR in 23 newly diagnosed Type-1 diabetic patients and 32 healthy subjects.

T and non-T cells were purified by rosetting mononuclear cells with sheep erythrocytes and separating the rosetted T cells from the nonrosetted non-T cells by density gradient centrifugation. Purity of T-lymphocytes isolated was 90% as determined by indirect immunofluorescent analysis with monoclonal antibodies. Proliferation of lymphocytes was measured in response to phytohaemagglutinin and of concanavalin A in a lymphocyte transformation test.

In the present study, a deficient AMLR is demonstrated in patients with newly diagnosed Type-1 diabetes. Our data provide evidence for an aberrant immune regulation at the time of diabetes manifestation. The deficient AMLR may represent the in-vitro expression of an in-vivo process against pancreatic cells.

Key words

Type-1 diabetes - AMLR - Lymphocyte-Purification - Lymphocyte-Transformation - Lymphocyte-Phenotyping