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Exp Clin Endocrinol Diabetes 1991; 98(4): 32-36
DOI: 10.1055/s-0029-1211097
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Simple Determination of Iodine in Small Specimens of Thyroid Tissue1)

Beate Tiran, O. Wawschinek, O. Eber* , A. Beham, S. Lax** , Marjan Dermelj***
  • Institute of Medical Biochemistry (Head: Prof. Dr. A. Holasek)
  • * University of Graz, Hospital Krankenhaus der Barmherzigen Brüder (Head: Prof. Dr. O. Eber), Graz-Eggenberg
  • ** Institute of Pathological Anatomy (Head: Prof. Dr. H. Denk), University of Graz, Graz/Austria
  • *** Nuclear Chemistry Department, E. Kardelj University, Ljubljana/Yugoslavia
1) Dedicated to Prof. Dr. A. Holasek on the occasion of his 70th birthday
Further Information

Publication History

1990

Publication Date:
16 July 2009 (online)

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Summary

This work describes a simple photometric determination of the iodine concentration in thyroid tissue, a method based on the well-known catalytic Sandell-Kolthoff reaction. The modified eerie arsenite reaction is very sensitive and does not require complicated laboratory equipment. It can be performed in any routine clinical laboratory if instructions are followed strictly. By means of this method iodine concentration can be determined even in small specimens, for instance puncture samples of thyroid tissue or thyroid glands of small animals (mouse). Previous to the analysis, the tissue is digested in a mixture of sodium chlorate and perchloric acid at 100°C. Using this manner of digestion between 94 and 110% of iodine in the sample were recovered. Comparison with neutron activation analysis showed excellent agreement of the obtained values.

Key words

Iodine - Microdetermination - Thyroid tissue - Sandell-Kolthoff reaction