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Exp Clin Endocrinol Diabetes 1992; 99(2): 77-79
DOI: 10.1055/s-0029-1211139
Original

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Effects of Acetylcholine on the Release of Thyrotropin-Releasing Hormone from the Rat Retina in vitro

T. Mitsuma* , Y. Hirooka* , M. Kayama* , K. Izeki* , T. Nogimori**
  • * Fourth Department of Internal Medicine, Aichi Medical University, Nagakute, Aichi
  • ** Department of Internal Medicine, Konanshowa Hospital, Konan, Aichi/Japan
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Publikationsverlauf

1991

Publikationsdatum:
15. Juli 2009 (online)

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Summary

Effects of acetylcholine on the release of thyrotro-pin-releasing hormone (TRH) from the rat retina were studied in vitro. The retina was incubated in medium 199 (pH 7.4) with 1.0mg/ml of bacitracin and 100 µg/ml of ascorbic acid (medium) for 20 min. The amount of TRH release into the medium was measured by radioimmunoassay.

The TRH release from the rat retina was enhanced significantly in a dose-related manner with the addition of acetylcholine and inhibited with addition of atropine. The stimulatory effect of acetylcholine on TRH release from the retina was blocked with the addition of atropine. The elution profile of methanol-extract of the rat retina was identical to that of synthetic TRH.

The findings suggest that the cholinergic system stimulates TRH release from the rat stomach in vitro.

Key words

TRH - Retina - Acetylcholine - Rat