Type II Iodothyronine 5′-Deiodinase in Mouse Brown Adipose Tissue is Stimulated by a Single Injection of Isoproterenol in an Oil/Water Emulsion

Summary

A single subcutaneous morning injection of the β-adrenergic agonist isoproterenol (ISO) (10 mg/kg body weight) in an oil/water emulsion (70/30; v/v) caused a marked increase in the activity of the enzyme type II iodothyronine 5′-deiodinase (5′-D II) in the interscapular brown adipose tissue of BDF-1 mice. After a delay of 4 hours, the 5′-D II activity began to rise in an almost linear fashion and was increased 3-fold after 8 hours, when compared to the control values. The results indicate that this method of ISO administration may be a valid tool for artificial stimulation of brown adipose tissue in animals by β-adrenergic agonists.

Key words

Brown fat - Isoproterenol - β-adrenergic receptor - Type II Iodothyronine 5′-Deiodinase