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Exp Clin Endocrinol Diabetes 1994; 102(6): 455-459
DOI: 10.1055/s-0029-1211318
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© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Immunohistochemical studies on specificity and affinity of the BB5-antibody against human parathyroid tissue

U. Joseph1 , 2 , H. G. Seesko1 , K. Neumann3 , W. Canee4 , S. Wells4 , M. Rothmund1
  • 1Klinik für Allgemeinchirurgie, Klinikum der Philipps Universität Marburg, USA
  • 2Medizinische Poliklinik, Klinikum der Philipps Universität Marburg, USA
  • 3Abteilung für allgemeine Pathologie, Klinikum der Philipps Universität Marburg, USA
  • 4Department of Surgery Washington University School, St. Louis, Mo., USA
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Publication History

Publication Date:
15 July 2009 (online)

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Summary

Localisation of parathyroid tissue in hyperparathyroidism may be difficult with standard methods. Immunoscintigraphy, using radiolabeled antibodies against epitopes of human parathyroid cells, could be a promising alternative. Therefore, we studied the necessary preconditions, whether the so-called BB5-antibody, directed against parathyroid cell membranes possesses sufficient specificity and affinity to be employed in immunoscintigraphy. Specificity was tested immunohisto-chemically with APAAP-staining of 39 different human tissues. Additionally, an immunoscore-based quantitative comparison was performed to test the affinity of BB5-antibody for normal and pathologic parathyroid tissue.

Specificity was proven by the fact that of all 39 tested tissue-types only the parathyroid tissue was BB5-posi-tive. Normal parathyroid tissue showed a significantly higher affinity to the BB5-antibody than pathologic parathyroid tissue (p < 0.03). However, all tissue samples from primary or secondary hyperparathyroidism exhibited sufficient staining.

We conclude that the BB5-antibody fulfills the necessary conditions to be tried for immunoscintigraphical localisation of the parathyroid glands.

Key words

Parythyroid - monoclonal antibody - immuno-histochemistry