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Exp Clin Endocrinol Diabetes 1995; 103: 71-73
DOI: 10.1055/s-0029-1211397
Molecular Biology of Islets of Langerhans

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Overexpression of Calcium/Calmodulin-Dependent Protein Kinase II in Insulinoma Cells by use of a Retroviral Vector

M. Möhlig, P. Mayer, P. A. Horn, H. Schatz, A. Pfeiffer
  • Medizinische Klinik und Poliklinik, Berufsgenossenschaftliche Krankenanstalten Bergmannsheil, Ruhr-Universität, Bochum, Germany
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Publication History

Publication Date:
15 July 2009 (online)

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Summary

The manner in which increasing intracellular calcium levels lead to insulin exocytosis is not known. Possibly the signal is mediated by activation of calcium/ calmodulin-dependent protein kinase II (CaM Kin II). In this work the establishment of an insulinoma cell line stably overexpressing CaM Kinase II subtype delta 2 by an ecotropic retroviral transduction system is described.

Key words

Calmodulin dependent protein kinases - antibody - retrovirus - transfection