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Exp Clin Endocrinol Diabetes 2009; 117(8): 423-431
DOI: 10.1055/s-0029-1214385
Article

© J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Reactivity to Helicobacter pylori Antigens in Patients Suffering from Thyroid Gland Autoimmunity

K. Stechova1 , R. Pomahacova2 , J. Hrabak3 , M. Durilova1 , J. Sykora2 , D. Chudoba4 , V. Stavikova1 , K. Flajsmanova1 , J. Varvarovska2
  • 1Department of Paediatrics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic
  • 2Department of Paediatrics, Faculty of Medicine Plzen, Charles University, Prague, Czech Republic
  • 3Department of Microbiology, Faculty of Medicine Plzen, Charles University, Prague, Czech Republic
  • 4Department of Biology and Genetics, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic
Further Information

Publication History

received 10.10.2008 first decision 22.01.2009

accepted 24.02.2009

Publication Date:
26 May 2009 (online)

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Abstract

The role of infection in autoimmunity is widely discussed. In this study we concentrated on relationship between Helicobacter pylori as a very important gastroduodenal pathogen and autoimmune thyroiditis (AT). Forty seven AT patients and 34 healthy controls were enrolled. They were split into: THP (H.pylori positive patients, n=17), THN (H.pylori negative patients, n=30), CP (H.pylori positive controls, n=17) and CN groups (H.pylori negative controls, n=17). By protein microarray we analysed production of 23 cytokines and chemokines prior and post stimulation with H.pylori lysate and its lipopolysacharide (LPS). Reactivity to lysate as well as to bacterial LPS differed within groups. The lowest basal cytokine and chemokine production was observed in CN group but these subjects reacted significantly to specific stimulation by increasing IFN-gamma (in comparison with THP p=0.01 for LPS and p=0.004 for H.pylori lysate) and TGF-beta production (p=0.015 for LPS). In contrast, IL-10 and IL-5 were decreased in this group. In CP, THN and THP groups, we observed in general higher chemokine response. THP group increased proinflammatory IL-6 after specific stimulation as well (in comparison with CP p<0.0001 for LPS stimulation). We observed different “reactivity pattern” to H.pylori within groups with low basal cytokine and chemokine production in healthy H.pylori negative controls but with clear specific response in IFN-gamma and TGF-beta production in this group. Adequate immune reaction which is joined to appropriate immunoregulation leads to prevention of the chronic infection and on the other hand may prevent the development of “connected” diseases such as autoimmune.

Key words

Helicobacter pylori - autoimmune thyroiditis - cytokine - chemokine

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Correspondence

K. StechovaMD, PhD 

Laboratory of Autoimmune Diseases

Department of Paediatrics

University Hospital Motol

V Uvalu 84

150 06

Prague 5 – Motol

Czech Republic

Phone: +420/224/432 089

Fax: +420/224/432 020

Email: info@labao.cz