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DOI: 10.1055/s-0029-1216952
Synthesis of an Arginine Tagged [Cys155-Arg180] Fragment of NY-ESO-1: Elimination of an Undesired By-product Using ‘In House’ Resins
Publication History
Publication Date:
21 August 2009 (online)
Abstract
During the solid-phase synthesis of a peptide fragment derived from the cancer protein NY-ESO-1 incorporating a solubilising arginine tag, a significant by-product was obtained that lacked the arginine tag. The formation of this by-product (and others) was highly dependant on the quality of the resin used and was completely removed when the resin was synthesized ‘in house’.
Key words
solid-phase - resins - HMBA linker - arginine tag
- Supporting Information for this article is available online:
- Supporting Information
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References
Harris, P. W. R.; Brimble, M. A. manuscript in preparation.
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23According to reference 2, resins that swell less than 7 mL/g in CH2Cl2 should be rejected. For commercially obtained Wang, Rink Amide AM, and 2-Cl trityl reins used here, the swelling was 6.1, 5 and 4.5 mL/g, respectively, while for the ‘in house’ reins 16 and 17, the swelling was 9 and 9.7 mL/g, respectively.