Int J Sports Med 2009; 30(9): 684-690
DOI: 10.1055/s-0029-1224176
Immunology

© Georg Thieme Verlag KG Stuttgart · New York

Exercise Reduces Effects of Creatine on Lung

R. P. Vieira 1 , 2 , A. C. Duarte 2 , A. B. G. Santos 3 , M. C. R. Medeiros 3 , T. Mauad 3 , M. A. Martins 4 , C.R. F. Carvalho 2 , M. Dolhnikoff 3
  • 1Albert-Ludwigs University of Freiburg, Pneumology, Freiburg, Germany
  • 2University of Sao Paulo, School of Medicine, Department of Physical Therapy (LIM 34), Sao Paulo, Brazil
  • 3University of Sao Paulo, School of Medicine, Department of Pathology (LIM 05), Sao Paulo, Brazil
  • 4University of Sao Paulo, School of Medicine, Department of Medicine (LIM 20), Sao Paulo, Brazil
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Publikationsverlauf

accepted after revision March, 30 2009

Publikationsdatum:
30. Juni 2009 (online)

Abstract

We recently demonstrated that creatine supplementation increased some features of lung allergic sensitization in mice. On the other hand, other studies have shown that aerobic exercise inhibited allergic airway inflammation and remodeling. We hypothesized that aerobic exercise may decrease the exacerbatory effects of the creatine supplementation in a murine model of asthma. Balb/c mice were divided into six groups: Control, Creatine (Cr), Low Intensity Exercise+Creatine (Low+Cr), Ovalbumin (OVA), Ovalbumin+Creatine (OVA+Cr) and Ovalbumin+Creatine+Low Intensity Exercise (OVA+Cr+Low). OVA-sensitized groups were sensitized with OVA intraperitoneal injections (days 0, 14, 28, and 42). Aerosol challenge (OVA 1%) and Cr treatment (0.5 g/kg/day) were initiated on Day 21 until Day 53. Low intensity exercise began on day 22 and was sustained until day 50. Low intensity exercise in the presence of creatine supplementation in sensitized mice resulted in a decreased number of eosinophils in BALF (p<0.001) and in the airways (p<0.001), and a decreased density of inflammatory cells positive to IL-4 (p<0.001) and IL-5 (p<0.001), airway collagen (p<0.001) and elastic fibers (p<0.001) content, airway smooth muscle thickness (p<0.001) and bronchoconstriction index (p<0.05) when compared with OVA+Cr group. These results suggest that aerobic exercise reduces the exacerbatory effects of creatine supplementation in chronically sensitized mice.

References

  • 1 Becque MD, Lochmann JD, Melrose DR. Effects of oral creatine supplementation on muscular strength and body composition.  Med Sci Sports Exerc. 2000;  32 654-658
  • 2 Brusselle GG, Kips JC, Tavernier JH, van der Heyden JG, Cuvellier CA, Pauwelss RA, Bluethmann H. Attenuation of allergic airway inflammation in IL-4 deficient mice.  Clin Exp Allergy. 1994;  24 73-80
  • 3 Dorsch KD, Bell A. Dietary supplement use in adolescents.  Curr Opin Pediatr. 2005;  17 653-657
  • 4 Ellis AC, Rosenfeld J. The role of creatine in the management of amyotrophic lateral sclerosis and other neurodegenerative disorders.  CNS Drugs. 2004;  18 967-980
  • 5 Fanelli A, Cabral ALB, Neder JA, Martins MA, Carvalho CRF. Exercise training on disease control and quality of life in asthmatic children.  Med Sci Sports Exerc. 2007;  39 1481-1486
  • 6 Fu CL, Chuand YH, Chau LY, Chiang BL. Effects of adenovirus-expressing IL-10 in alleviating airway inflammation in asthma.  J Gene Med. 2006;  8 1393-1399
  • 7 Fuld JP, Kilduff LP, Neder JA, Pitsiladis Y, Lean MEJ, Ward SA, Cotton MM. Creatine supplementation during pulmonary rehabilitation in chronic obstructive pulmonary disease.  Thorax. 2005;  60 531-537
  • 8 Idzko M, Hammad H, van Nimwegen M, Kool M, Willart MAM, Muskens F, Hoogsteden HC, Luttmann W, Ferrari D, Di Virgilio F, Virchow  Jr  JC, Lambrecht BN. Extracellular ATP triggers and maintains asthmatic airway inflammation by activating dendritic cells.  Nat Med. 2007;  13 913-919
  • 9 Ipsiroglu OS, Stromberger C, Ilas J, Hoger H, Muhl A, Stockler-Ipsiroglu S. Changes of tissue creatine concentrations upon oral supplementation of creatine-monohydrate in various animal species.  Life Sci. 2001;  69 1805-1815
  • 10 Kreis R, Kamber M, Koster M, Felblinger J, Slotboom J, Hoppeler H, Boesch C. Creatine supplementation – Part II: in vivo magnetic resonance spectroscopy.  Med Sci Sports Exerc. 1999;  31 1770-1777
  • 11 Leigh R, Ellis R, Wattie JN, Hirota JA, Matthaei KI, Foster PS, O’Byrne PM, Inman MD. Type 2 cytokines in the pathogenesis of sustained dysfunction and airway remodeling in mice.  Am J Respir Crit Care Med. 2004;  169 860-867
  • 12 Maughan RJ, King DS, Lea T. Dietary supplements.  J Sports Sci. 2004;  22 95-113
  • 13 Moldoveanu AI, Shephard RJ, Shek PN. The cytokine response to physical activity and training.  Sports Med. 2001;  31 115-144
  • 14 Nakagome K, Dohi M, Okunishi K, Komagata Y, Nagatani K, Tanaka R, Miyazaki J, Yamamoto K. In vivo IL-10 gene delivery suppresses airway eosinophilia and hyperreactivity by down-regulating apc functions and migration without impairing the antigen-specific systemic immune response in a mouse model of allergic airway inflammation.  J Immunol. 2005;  174 6955-6966
  • 15 Nomura A, Zhang M, Sakamoto T, Ishii Y, Morishima Y, Mochizuki M, Kimura T, Uchida Y, Sekizawa K. Anti-inflammatory activity of creatine supplementation in endothelial cells in vitro.  Br J Pharmacol. 2003;  139 715-720
  • 16 Oopik V, Paasuke M, Timpmann S, Medijainen L, Ereline J, Gapejeva J. Effects of creatine supplementation during recovery from rapid body mass reduction on metabolism and muscle performance capacity in well-trained wrestlers.  J Sports Med Phys Fitness. 2002;  42 330-339
  • 17 Pastva A, Estell K, Schoeb TR, Atkinson TP, Schwiebert LM. Aerobic exercise attenuates airway inflammatory responses in a mouse model of atopic asthma.  J Immunol. 2004;  172 4520-4526
  • 18 Pastva A, Estell K, Schoeb TR, Schwiebert LM. RU486 blocks the anti-inflammatory effects of exercise in a murine model of allergen-induced pulmonary inflammation.  Brain Behav Immun. 2005;  19 413-422
  • 19 Petersen AMW, Pedersen BK. The anti-inflammatory effect of exercise.  J Appl Physiol. 2005;  98 1154-1162
  • 20 Pucar D, Dzeja PP, Bast P, Juranic N, Macura S, Terzic A. Cellular energetics in the preconditioned state: protective role for phosphotransfer reactions captured by 18O-assisted 31P NMR.  J Biol Chem. 2001;  276 44812-44819
  • 21 Ram FS, Robinson SM, Black PN, Picot J. Physical training for asthma.  Cochrane Database Syst Rev. 2005;  ((4)) CD001116
  • 22 Randolph CC. Allergic rhinitis and asthma in the athlete.  Allergy Asthma Proc. 2006;  27 104-109
  • 23 Saks VA, Stepanov V, Jaliashvili IV, Konerev EA, Kryzhanovsky SA, Strumia E. Molecular and cellular mechanisms of action for the cardioprotective and therapeutic role of creatine phosphate. In: Conway MA, Clark JF. (eds). Creatine and Creatinine Phosphate: Scientific and Clinical Perspectives. San Diego: Academic Press 1996: 91-114
  • 24 Santos RV, Bassit RA, Caperuto EC, Costa Rosa LF. The effect of creatine supplementation upon inflammatory and muscle soreness markers after a 30 km race.  Life Sci. 2004;  75 1917-1924
  • 25 Spicuzza L, Di Maria G, Polosa R. Adenosine in the airways: implications and applications.  Eur J Pharmacol. 2006;  533 77-88
  • 26 Tilz GP, Domej W, Diez-Ruiz A, Weiss G, Brezinschek R, Brezinschek HP, Huttl E, Pristautz H, Wachter H, Fuchs D. Increased immune activation during and after physical exercise.  Immunobiology. 1993;  188 194-202
  • 27 Vieira RP, Claudino RC, Duarte ACS, Santos ABG, Perini A, Faria Neto HCC, Mauad T, Martins MA, Dolhnikoff M, Carvalho CRF. Aerobic exercise decreases chronic allergic lung inflammation and airway remodeling in mice.  Am J Resp Crit Care Med. 2007;  176 871-877
  • 28 Vieira RP, Duarte ACS, Claudino RC, Perini A, Santos ABG, Moriya HT, Arantes-Costa FM, Martins MA, Carvalho CRF, Dolhnikoff M. Creatine supplementation exacerbates allergic lung inflammation and airway remodeling in mice.  Am J Resp Cell Mol Biol. 2007;  37 660-667
  • 29 Vieira RP, Andrade VF, Duarte ACS, Santos ABG, Mauad T, Martins MA, Dolhnikoff M, Carvalho CRF. Aerobic conditioning and allergic pulmonary inflammation in mice II: Effects on lung vascular and parenchymal inflammation and remodeling.  Am J Physiol. 2008;  295 L670-L679
  • 30 Webb DC, McKenzie AN, Koskinen AM, Yang M, Mattes J, Foster PS. Integrated signals between IL-13, IL-4, and IL-5 regulate airways hyperreactivity.  J Immunol. 2000;  165 108-113
  • 31 Zoladz JA, Korzeniewski B, Grassi B. Training-induced acceleration of oxygen uptake kinetics in skeletal muscle: the underlying mechanisms.  J Physiol Pharmacol. 2006;  57 (Suppl (10)) 67-84

Correspondence

Dr. R. P. Vieira

Albert-Ludwigs University of Freiburg

Pneumology

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79106

Freiburg

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