Planta Med 2009; 75 - PA74
DOI: 10.1055/s-0029-1234399

A new flavonol with anti-inflammatory activity from Boldoa purpurascens Cav.

DM González Mosquera 1, A Kilonda 2, S Toppet 2, F Compernolle 2, W Dehaen 2, S Apers 3, L Pieters 3, A Cuéllar Cuéllar 4, L Vicet Muro 1, Y Hernández Ortega 1
  • 1Department of Pharmacy at Marta Abreu University, Road to camajuani Km 5 Santa Clara Cuba
  • 2Molecular Design and Synthesis, K.U.Leuven, Celestijnenlaan 200F, 3001 Leuven-Heverlee, Belgium
  • 3Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, Belgium
  • 4Institute for Pharmacy and Food (IFAL), University of Havana, Ave. 23, 21425, Lisa, C. Habana, Cuba

Phytochemical analysis of the leaves of Boldoa purpurascens Cav. [1] led to the isolation of two flavonol glycosides [2,3]. The structure of the new compound was determined by mass spectrometry and by 1D and 2D NMR analysis as 4',5-dihydroxy-6,7-methylenedioxyflavonol-3-O-α-L-rhamnopyranosyl-(1–2)-β-D-xylopyranoside [4].The aglycone 3,4',5-dihydroxy-6,7-methylenedioxyflavonol is known as gomphrenol [5]. The new flavonoid was evaluated for its effects in the acute and chronicle phases of inflammation. For this reason, two experimental techniques were developed: edema induced by dextrans, and granulomas induced by cotton disks. Test doses of 2.5, 5.0 and 10mg/Kg of weight were used, with a volume of administration of 10 mL/Kg. Indomethacin was the positive control (7 and 5mg/Kg) and distilled water the negative control. Gonfrenol-3-O– [ α- L-rhamnosyl-(1–2) –β- D-xilósido] presented anti-inflammatory activity in the acute phase to superior dose to 2.5mg/Kg in the experimental pattern of edema to plant induced by dextrano; also, this activity increased when increasing the dose. In the granulomas pattern induced by cotton disks, the compound presented similar anti-inflammatory activity to the Indometacine to all the evaluated doses, being bigger the effect to the 10mg/Kg dose. The statistical analysis was carried out by the test of Kruskal-Wallis with an interval of trust of 99% and a level of significance of 0.816. The flavonol; showed significant dose-dependent inhibition of both acute and chronic inflammation. The activity was comparable to that of the standard reference drug, indometacine. The results of the present investigation indicated that the flavonol isolated of B purpurascens Cav. shows profound antiinflammatory activity.

Keywords: Boldoa purpurascens, Nyctaginaceae, flavonol glycosides, gomphrenol glycosides.

Reference 1. Roig, J.T (1988) Dictionary of Cuban Common Yams, 3rd. Editorial Science and Technic

2. Niassy, B. et al. (2004) C. R. Chimie 7: 993–996.

3. Jpn. Kokai Tokkyo Koho (1988) JP63203682 A2.

4. Magalhães, A.F. et al. (2007) Acad. Bras. Cienc. 79:351–367.

5. Bouillant, M.L. et al. (1978) Phytochemistry 17:2138–40.