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Planta Med 2010; 76(7): 694-696
DOI: 10.1055/s-0029-1240623
Pharmacology
Letters
© Georg Thieme Verlag KG Stuttgart · New York

Green Tea Catechins: Inhibitors of Glycerol-3-Phosphate Dehydrogenase

Chung-Cheng Kao1 [*] , Bo-Tsung Wu2 [*] , Yi-Wei Tsuei1 , Li-Jane Shih3 , Yu-Liang Kuo4 , Yung-Hsi Kao2
  • 1Department of Emergency, Armed Forces Taoyuan General Hospital, Taoyuan, Taiwan
  • 2Department of Life Sciences, National Central University, Jhongli, Taiwan
  • 3Department of Joint Laboratory, Armed Forces Taoyuan General Hospital, Taoyuan, Taiwan
  • 4Department of Obstetrics and Gynecology, Armed Forces Taoyuan General Hospital, National Defense Medical Center, Taoyuan, Taiwan
Further Information

Publication History

received August 22, 2009 revised October 25, 2009

accepted October 28, 2009

Publication Date:
20 November 2009 (online)

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Abstract

Green tea catechins, especially (−)-epigallocatechin-3-gallate (EGCG), are known to regulate obesity and fat accumulation. We performed a kinetic analysis in a cell-free system to determine the mode of inhibition of glycerol-3-phosphate dehydrogenase (GPDH; EC 1.1.1.8) by EGCG. GPDH catalyzes the β-nicotinamide adenine dinucleotide (NADH)-dependent reduction of dihydroxyacetone phosphate (DHAP) to yield glycerol-3-phosphate, which serves as one of the major precursors of triacylglycerols. We found that EGCG dose-dependently inhibited GPDH activity at a concentration of approximately 20 μM for 50 % inhibition. The IC50 values of other green tea catechins, such as (−)-epicatechin, (−)-epicatechin-3-gallate, and (−)-epigallocatechin, were all above 100 µM. This suggests a catechin type-dependent effect. Based on double-reciprocal plots of the kinetic data, EGCG was a noncompetitive inhibitor of the GPDH substrates, NADH and DHAP, with respective inhibition constants (Ki) of 18 and 31 µM. Results of this study possibly support previous studies that EGCG mediates fat content.

Key words

green tea - Camellia sinensis - Theaceae - epigallocatechin‐3‐gallate

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1 C.-C. K. and B.-T. W. contributed equally to this work.

Dr. Yung-Hsi Kao

Department of Life Science
National Central University

Number 300, Jhongda Road

Jhongli City, Taoyuan County 32001

Taiwan

Phone: + 88 6 34 26 08 39

Fax: + 88 6 34 22 84 82

Email: ykao@cc.ncu.edu.tw

    www.thieme-connect.de/ejournals/toc/plantamedica