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Planta Med 2010; 76(6): 648-651
DOI: 10.1055/s-0029-1240632
Biochemistry, Molecular Biology and Biotechnology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Authentication of the Oriental Medicinal Plant Ligusticum tenuissimum (Nakai) Kitagawa (Korean Go-Bon) by Multiplex PCR

Baigalmaa Jigden1 , Hongtao Wang1 , Myung Kyum Kim1 , Yeon-Ju Kim1 , Jun Gyo In1 , Deok-Chun Yang1
  • 1Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Giheung-gu Yongin-si, Gyeonggi-do, South Korea
Further Information

Publication History

received March 13, 2009 revised July 10, 2009

accepted October 27, 2009

Publication Date:
25 November 2009 (online)

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Abstract

The oriental medicinal plant Ligusticum tenuissimum (Korean name, Go-Bon) is widely used in Korea and China. L. tenuissimum (Go-Bon) has been employed in the treatment of headache and common cold, and as a fever remedy. The internal transcribed spacer (ITS) region was sequenced from thirty-four Go-Bon samples collected from botanical gardens and markets in Korea and China to identify and authenticate L. tenuissimum. Based on the ITS sequences, the thirty-four Go-Bon samples were classified into three groups: L. tenuissimum (Korean Go-Bon), L. jeholense (Chinese Go-Bon), and unknown Chinese Ligusticum species. Three specific primers were designed to identify the three groups of Ligusticum species using multiplex PCR. The established multiplex-PCR was proved to be effective for the differentiation of L. tenuissimum in commercial plant materials.

Key words

internal transcribed sequence (ITS) - Apiaceae - Ligusticum tenuissimum - Ligusticum jeholense - multiplex PCR - single nucleotide polymorphism (SNP)

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Deok Chun Yang

Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank
Kyung Hee University

Seocheon-dong 1

Giheung-gu Yongin-si

Gyeonggi-do 449–701

South Korea

Phone: + 82 3 12 01 26 88

Fax: + 82 3 12 02 26 87

Email: deokchunyang@yahoo.co.kr