Abstract
Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein,
and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects.
In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA)
for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal
antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.05–6.25 µg/mL.
Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with
those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA
is a precise, accurate, and sensitive method for the determination of total isoflavonoids
in P. candollei.
Key words
daidzin - enzyme‐linked immunosorbent assay (ELISA) - isoflavonoids - Leguminosae
-
Pueraria candollei
- puerarin
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Assoc. Prof. Waraporn Putalun
Faculty of Pharmaceutical Sciences
Khon Kaen University
Friendship Highway
40002 Khon Kaen
Thailand
Phone: + 66 43 20 23 78
Fax: + 664 3 20 23 79
Email: waraporn@kku.ac.th
