Abstract
This work was designed to develop a simple, effective, and reliable LC system to identify
a chemical marker and compare Sambucus nigra L. and Sambucus australis Cham. et Schltdl. flower extracts (American and European elder). Rutin was the main
constituent of both species. The developed method showed a linear response in the
range of 10 to 45 µg · mL−1 for rutin and 1.75 to 3.25 µg · mL−1 for samples of the Sambucus species. Precision was determined and the relative standard deviations were 1.75 %
for HSN and 1.28 % for HSA for intraday precision and 1.28 % and 1.51 % for inter-day
precision, respectively, while accuracy was 97.9 % for HSN and 99.41 % for HSA. Quantification
and detection limits as well as robustness were determined, presenting adequate results.
The LC method showed an adequate performance for the separation of flavonoid glycosides
in S. nigra and S. australis extracts, since the presence of interference had been previously evaluated. The analysis
of thirty different samples of S. nigra and S. australis of different origins did not show significant variability among them. An accelerated
stability study revealed a significant decrease in the first 30 days reaching 57 %
in 90 days for S. australis samples and a total decrease of 25 % in 90 days for S. nigra samples, considering rutin as the chemical marker. These results will contribute
to quality control analysis routines of these raw materials in pharmaceutical production
facilities.
Key words
Sambucus nigra
-
Sambucus australis
- Caprifoliaceae - stability study - flavonoids - HPLC
References
Sambucus nigra .
Plant Med Phytother.
1983;
17
173-181
Sambucus nigra L.
Ann Pharm Fr.
1991;
49
258-262
Sambucus nigra ).
Phytochemistry.
1964;
3
267-268
Sambucaceae. Burkart A Flora Ilustrada de Entre Rios (Argentina), Caprifoliaceae. Buenos Aires;
INTA 1974: 50-52 Sambucus nigra L. e Sambucus australis Cham. Et Schlecht.
Rev Bras Farm.
1990;
71
88-90
Sambucus (Caprifoliaceae) utilizadas como medicinais no Brasil. Parte I. Sambucus nigra L.
Rev Bras Farmacogn.
2007;
17
249-261
Sambucus (Caprifoliaceae) utilizadas como medicinais no Brasil. Parte II. Sambucus australis Cham. & Schltdl.
Rev Bras Farmacogn.
2007;
17
414-425
Calendula officinalis and Sambucus nigra by high-performance liquid and micellar electrokinetic capillary chromatography.
J Chromatogr.
1992;
593
165-170
Calluna vulgaris, Sambucus nigra , and Vaccinium myrtillus .
J Agric Food Chem.
2008;
56
9080-9086
Sambucus nigra L.) genotypes best suited for the preparation of elderflower extracts rich in flavonoids
and phenolic acids.
Eur Food Res Technol.
2008;
227
293-305
http://www.ich.org accessed 2009
amélia@farmacia.ufrgs.br
