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Planta Med 2010; 76(13): 1468-1472
DOI: 10.1055/s-0029-1240940
Biochemistry, Molecular Biology and Biotechnology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Enhancement of Artemisinin Content through Four Cycles of Recurrent Selection with Relation to Heritability, Correlation and Molecular Marker in Artemisia annua L.

Shilpi Paul1 , Suman P. S. Khanuja1 , Ajit Kumar Shasany1 , Madan Mohan Gupta1 , Mahendra P. Darokar1 , Dharmendra Saikia1 , Anil Kumar Gupta1
  • 1Central Institute of Medicinal and Aromatic Plants, Lucknow, India
Further Information

Publication History

received January 21, 2009 revised Dec. 17, 2009

accepted February 1, 2010

Publication Date:
18 March 2010 (online)

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Erratum for: Enhancement of Artemisinin Content through Four Cycles of Recurrent Selection with Relation to Heritability, Correlation and Molecular Marker in Artemisia annua L.Planta Med 2010; 76(13): E5-E5
DOI: 10.1055/s-0030-1249828

Abstract

Due to the high demand and low yield of the anti-malarial drug artemisinin in natural populations of Artemisia annua (Quinghao), an attempt has been made to enhance the artemisinin content through 4 cycles of recurrent selection (C0-C3) using selected genotypic and phenotypic traits. Based on their phenotypic and genotypic characteristics, the top 5 % plants of each cycle were selected, and their seedlings were planted in poly-cross block to produce seeds for the subsequent generation. A significant increase in the artemisinin content (0.15 % in C0 to 1.16 % in C3, i.e., about 40 % genetic gain over the generation) was observed. This enhancement was directly correlated with the plant height and branching intensity in all four cycles. Similarly, the PCV (phenotypic coefficient of variation) and GCV (genotypic coefficient of variation) have been observed to have a higher value for artemisinin content. The DNA marker (MAP 12) with relation to artemisinin was also identified for high yielding genotypes in all four cycles of selection. Over the four cycles of recurrent selection, the plant developed an oval appearance (Variety: CIM-Arogya) and a high artemisinin content (1.16 %).

Key words

Artemisia annua - Asteraceae - artemisinin - recurrent selection - correlation - molecular marker

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Dr. Shilpi Paul, Scientist

G. B. Pant Institute of Himalayan Environment and Development

Kosi-Katarmal

Almora 263643

Uttaranchal

India

Phone: + 91 59 62 24 10 41, 24 11 54

Fax: + 91 59 62 24 10 14, 24 11 50

Email: shilpipaul01@yahoo.co.in