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Planta Med 2010; 76(14): 1525-1529
DOI: 10.1055/s-0029-1241003
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Fo Shou San, an Ancient Herbal Decoction Prepared from Rhizoma Chuanxiong and Radix Angelicae Sinensis, Stimulates the Production of Hemoglobin and Erythropoietin in Cultured Cells

Cathy Wen Chuan Bi1 , Heidi Qun Hui Xie1 , Li Xu1 , Jun Li1 , Anna Wing Han Cheung1 , Judy Ting Ting Zhu1 , Yu Zhong Zheng1 , Vicky Ping Chen1 , David Tai Wai Lau1 , Roy Chi Yan Choi1 , Tie Jie Wang1 , 2 , Tina Ting Xia Dong1 , Karl Wah Keung Tsim1
  • 1Department of Biology and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Hong Kong, PR China
  • 2Shenzhen Municipal Institute for Drug Control, Shenzhen, PR China
Weitere Informationen

Publikationsverlauf

received Sept. 24, 2009 revised February 8, 2010

accepted February 17, 2010

Publikationsdatum:
22. März 2010 (online)

Auch verfügbar auf
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Abstract

Fo Shou San (FSS) is an ancient herbal decoction comprised of Rhizoma Chuanxiong (RC; Chuanxiong) and Radix Angelicae Sinensis (RAS; Danggui) in a ratio of 2 : 3. It is mainly prescribed for patients having a blood deficiency. This combination is considered the most popular herb pair among Chinese medicines; however, the rationale of having these two chemically similar herbs within the decoction has historically not been made clear. Here, we attempted to reveal the chemical and biological properties of this decoction as a means to deduce its mechanism of action. The effects of FSS were determined in different cell culture models. With respect to stimulation of blood circulation, FSS inhibited ADP-mediated platelet aggregation in a dose-dependent manner. In order to reveal the hematopoietic effect of this decoction, FSS was applied onto cultured K562 human leukemia cells and Hep3B human hepatocellular carcinoma cells. Application of FSS in cultured K562 cells inhibited cell proliferation and subsequently induced the production of hemoglobin. Additionally, the mRNA expression of erythropoietin (EPO) was induced in a dose-dependent manner when FSS was applied to Hep3B cells. The current results reveal the effects of FSS in different cell models, paving a direction for mechanistic studies.

Key words

Angelica sinensis - Ligusticum chuanxiong - Umbelliferae

References

  • 1 Ning L, Chen C X, Jin R M, Wu Y P, Zhang H G, Sun C L, Song C Q, Hu Z B. Effect of components of Dang-Gui-Bu-Xue decoction on hematopenia.  Chin J Chin Mater Med. 2002;  27 50-53
    PubMedSuche in Google Scholar
  • 2 Wu D Z, Song C Q, Hen Z F, Kong J L, Fan Y, Hu Z B. Effects of Angelicae sinensis decoction for supplementing blood on the cardiac function in myocardial ischemia reperfusion injury of rats.  Pharm Clin Chin Mater Med. 1999;  15 3-6
    PubMedSuche in Google Scholar
  • 3 Xu J, Li Y K, Yang Z Y, Shen W H. The study on the pharmacology of Fo Shou San.  Pharmacol Clin Study Tradit Chin Med. 1989;  5 1-3
    PubMedSuche in Google Scholar
  • 4 Lu Y, Hu Y L, Kong X F, Wang D Y. Selection of component drug in activating blood flow and removing blood stasis of Chinese herbal medicine formula for dairy mastitis by hemorheological method.  J Ethnopharmacol. 2008;  116 313-317
    CrossrefPubMedSuche in Google Scholar
  • 5 Zhang X Z, Sun B H, Wang Y C. The influence of Ligusticum chuanxiong on brain hemodynamics in hemicrania.  Chin J Integr Med Cardio/Cerebrovasc Dis. 2008;  6 580
    PubMedSuche in Google Scholar
  • 6 Yi T, Leung K S Y, Lu G H, Zhang H, Chan K. Identification and comparative determination of senkyunolide A in traditional Chinese medicinal plants Ligusticum chuanxiong and Angelica sinensis by HPLC coupled with DAD and ESI-MS.  Chem Pharm Bull. 2005;  53 1480-1483
    CrossrefPubMedSuche in Google Scholar
  • 7 Gao Q T, Choi R C Y, Cheung A W H, Zhu J T T, Li J, Chu G K Y, Duan R R, Cheung J K H, Jiang Z Y, Dong X B, Zhao K J, Dong T T X, Tsim K W K. Chinese Danggui Buxue Tang – a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor α in cultured MCF-7 cells.  FEBS Lett. 2007;  581 233-240
    CrossrefPubMedSuche in Google Scholar
  • 8 Dong T T X, Zhao K J, Gao Q T, Zhao N J, Zhu J T T, Li J, Duan R R, Cheung A W H, Tsim K W K. Chemical and biological assessment of a Chinese herbal decoction containing Radix Astragali and Radix Angelicae Sinensis: determination of drug ratio in having optimized properties.  J Agric Food Chem. 2006;  54 2767-2774
    CrossrefPubMedSuche in Google Scholar
  • 9 Sasaki R, Masuda S, Nagao M. Molecular erythropoietin: multiple physiological functions and regulation of biosynthesis.  Biosci Biotechnol Biochem. 2000;  64 1775-1793
    CrossrefPubMedSuche in Google Scholar
  • 10 Daghman N A, McHale C M, Savage G M, Price S, Winter P C, Maxwell A P, Lappin T R. Regulation of erythropoietin gene expression depends on two different oxygen-sensing mechanisms.  Mol Genet Metab. 1999;  67 113-117
    CrossrefPubMedSuche in Google Scholar
  • 11 Mark A, Goldberg G, Allison G, James M C, Bunn H F. The regulated expression of erythropoietin by two hepatoma cell line.  Proc Natl Acad Sci USA. 1987;  84 7972-7976
    CrossrefPubMedSuche in Google Scholar
  • 12 Woessmann W, Zwanzger D, Borkhardt A. ERK signaling pathway is differentially involved in erythroid differentiation of K562 cells depending on time and the inducing agent in erythroid differentiation and heme synthesis.  Cell Biol Int. 2004;  25 403-410
    PubMedSuche in Google Scholar
  • 13 Li C Y, Tsai W J, Damu A G, Lee E J, Dung W T S, Thang N X, Thanh L. Isolation and identification of anti-platelet aggregatory principles from the leaves of Piper iolot.  J Agric Food Chem. 2007;  55 9436-9442
    CrossrefPubMedSuche in Google Scholar
  • 14 Lozzio C B, Lozzio B B. Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome.  Blood. 1975;  45 321-334
    PubMedSuche in Google Scholar
  • 15 Housset M, Daniel M T, Degos L. Small doses of Ara-C in the treatment of acute myeloid leukaemia: differentiation of myeloid leukaemia cell.  Br J Haematol. 1982;  51 125-129
    CrossrefPubMedSuche in Google Scholar
  • 16 Zhang D Z, Cho E, Wong J M. A critical role for the co-repressor N-CoR in erythroid differentiation and heme synthesis.  Cell Res. 2007;  17 804-814
    CrossrefPubMedSuche in Google Scholar
  • 17 Avalos B R, Kaufman S E, Tomonaga M, Williams R E, Golde D W, Gasson J C. K562 cells produce and respond to human erythroid-potentiating activity.  Blood. 1988;  6 1720-1725
    PubMedSuche in Google Scholar
  • 18 Vengellur A, Phillips J M, Hogenesch J B, Lapres J J. Gene expression profiling of hypoxia signaling in human hepatocellular carcinoma cells.  Physiol Genomics. 2005;  22 308-318
    CrossrefPubMedSuche in Google Scholar
  • 19 Hervouet E, Pecina P, Demont J, Vojtiskova A, Simonnet H, Houstek J, Godinot C. Inhibition of cytochrome c oxidase subunit 4 precursor processing by the hypoxia mimic cobalt chloride.  Biochem Biophys Res Commun. 2006;  344 1086-1093
    CrossrefPubMedSuche in Google Scholar
  • 20 Liu X D, Xue Y Y, Xie L, Ji H F, Wang G J. Pharmacokinetic study of Danggui, Chuanxiong and their decoction.  J China Pharm Univ. 2003;  34 448-451
    PubMedSuche in Google Scholar
  • 21 Chen Z, Wu H, Zhang Y, Shu H. Effect of “Jia Wei Fo Shou San” on erythrocyte membrane calcium, zinc concentration in pregnant rats with asymmetrical intrauterine growth retardation induced by passive smoking.  J Tongji Med Univ. 1997;  17 218-220
    PubMedSuche in Google Scholar
  • 22 Shu H Y, Chen Z, Jiao X F. Effects of Jiaweifoshousan on erythrocyte membrane microviscosity and erythrocyte ultrastructure in rats.  Chin J Microcirculation. 1998;  8 3-5
    PubMedSuche in Google Scholar

Prof. Karl W. K. Tsim

Department of Biology and Center for Chinese Medicine
The Hong Kong University of Science and Technology

Clear Water Bay Road

Hong Kong

PR China

Telefon: + 85 2 23 58 73 32

Fax: + 85 2 23 58 15 59

eMail: botsim@ust.hk