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Horm Metab Res 2010; 42(4): 268-273
DOI: 10.1055/s-0029-1243638
Humans, Clinical

© Georg Thieme Verlag KG Stuttgart · New York

Visfatin/PBEF/Nampt and Resistin Expressions in Circulating Blood Monocytes are Differentially Related to Obesity and Type 2 Diabetes in Humans

M. Laudes1 , F. Oberhauser1 , D. M. Schulte1 , S. Freude1 , 2 , R. Bilkovski1 , 2 , J. Mauer2 , 3 , G. Rappl4 , H. Abken4 , M. Hahn5 , O. Schulz1 , W. Krone1 , 2
  • 1Department of Internal Medicine II and Centre of Molecular Medicine, Universität zu Köln, Köln, Germany
  • 2CECAD-Cluster of Excellence in Cellular Stress Responses in Aging Associated Diseases, Universität zu Köln, Köln, Germany
  • 3Institute of Genetics, Universität zu Köln, Köln, Germany
  • 4Department of Internal Medicine I, Universität zu Köln, Köln, Germany
  • 5Institute of Medical Statistics, Informatics and Epidemiology, Universität zu Köln, Köln, Germany
Further Information

Publication History

received 17.08.2009

accepted 08.12.2009

Publication Date:
20 January 2010 (online)

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Abstract

Low-grade inflammation is important in the development of obesity related pathologies such as insulin resistance and type 2 diabetes, and also cardiovascular disease. Visfatin/PBEF/Nampt and resistin are proinflammatory adipokines secreted from adipocytes, monocytes, and macrophages, and have been linked to atherosclerotic plaque formation, recently. The aim of the present study was to investigate if the expression of these molecules in circulating blood monocytes is altered in obese and/or type 2 diabetic human subjects. Monocytes were isolated by CD14-antibody based magnetic cell sorting from blood samples of 17 lean controls, 20 obese nondiabetic subjects, and 19 obese patients with type 2 diabetes. FACS analysis was performed to test purity of the cell preparations. Expression of the different adipokines was measured by multiplex real-time PCR on RNA-level. Visfatin/PBEF/Nampt was found to be very strongly expressed in monocytes, whereas resistin levels were significantly lower. Furthermore, visfatin/PBEF/Nampt expression was significantly upregulated in obese type 2 diabetic patients, whereas obese nondiabetics exhibited similar levels compared to lean controls, indicating that visfatin/PBEF/Nampt levels are related to type 2 diabetes rather than to obesity. In contrast, resistin expression displayed a different pattern being significantly increased in obese subjects compared to controls but not related to type 2 diabetes. These data suggest a differential role for these two proinflammatory adipokines in linking metabolic diseases to atherosclerosis with visfatin/PBEF/Nampt being more important in patients with type 2 diabetes and resistin in obese but nondiabetic human subjects.

Key words

inflammation - insulin resistance - atherosclerosis

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Correspondence

Dr. M. Laudes

Klinik II und Poliklinik für Innere Medizin

Zentrum für Molekulare Medizin

Universität zu Köln

Kerpner Straße 62

50924 Köln

Germany

Phone: +49/221/478 5481

Fax: +49/221/478 3107

Email: matthias.laudes@uk-koeln.de