Deltoid Muscle Characteristics in Wrestlers

Abstract

The purpose of the present study was to investigate the deltoid muscle characteristics of wrestlers. Nine Greco-Roman competitive male wrestlers (mean age 20.1±2.7 yrs, height 175±0.6 cm, weight 83.2±12.5 kg, years of training 7.6±2.7 yrs) participated in this study. Six male healthy sedentary students (mean age 21.2±0.9 yrs, height 180±0.3 cm, weight 80.1±9.4 kg) served as controls. Muscle fibre distribution, cross-sectional area (CSA), as well as satellite cells, myonuclei and capillary density per muscle fibre area were determined by immunohistochemistry. Myosin heavy chain MHC isoform composition of single fibres was determined with protein electrophoresis. Immunohistochemical analysis showed that muscle fibre distribution of the MHC I and IIA were significantly higher in wrestlers than in controls (p<0.05). Electrophoretic analysis of single fibres revealed a significantly higher proportion of fibres containing MHC I and IIC in wrestlers (p<0.05). The mean CSA of type IIA fibres and the number of myonuclei per type II was significantly higher in wrestlers (p<0.05). We also found that the number of satellite cells was 2.5 fold higher in wrestlers than in the control group. This study suggests that the observed muscle fibre profile in the deltoid of wrestlers may represent an adaptation based on the specific mechanical and biochemical demands of the long-term training in Greco-Roman wrestling.

MHC analysis - muscle biopsies - single fibres - immunohistochemistry - resistance training

References

• 1 Adams GR, Hather BM, Baldwin KM, Dudley GA. Skeletal muscle myosin heavy chain composition and resistance training.  J Appl Physiol. 1993;  74 911-915
• 2 Allen DL, Monke SR, Talmadge RJ, Roy RR, Edgerton VR. Plasticity of myonuclear number in hypertrophied and atrophied mammalian skeletal muscle fibers.  J Appl Physiol. 1995;  78 1969-1976
• 3 Brooke MH, Kaiser K. Three “myosin adenosine triphosphatase” systems: the nature of their pH lability and sulflhydryl dependence.  J Histochem Cytocem. 1970;  18 670-672
• 4 Charifi N, Kadi F, Féasson L, Costes F, Geyssant A, Denis C. Enhancement of microvessel tortuosity in the vastus lateralis muscle of old men in response to endurance training.  J Physiol. 2004;  554 559-569
• 5 Charifi N, Kadi F, Féasson L, Denis C. Effects of endurance training on satellite cell frequency in skeletal muscle of old men.  Muscle Nerve. 2003;  28 87-92
• 6 Christoulas K, Kesidis N, Metaxas T, Vrabas IS, Vamvakoudis E, Mandroukas K. Fiber type distribution, fiber size, and capillary density of deltoid and vastus lateralis muscles: influence of bodybuilding.  Osterreichisches J Sportmed. 2003;  3 6-10
• 7 Harber MP, Gallagher PM, Trautmann J, Trappe SW. Myosin heavy chain composition of single muscle fibers in male distance runners.  Int J Sports Med. 2002;  23 484-488
• 8 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research.  Int J Sports Med. 2009;  30 701-702
• 9 Hikida RS, Staron RS, Hagerman FC, Walsh S, Kaiser E, Shell S, Hervey S. Effects of high-intensity resistance training on untrained older men. II. Muscle fiber characteristics and nucleo-cytoplasmic relationships.  J Gerontol A Biol Sci Med Sci. 2000;  55 B347-B354
• 10 Howald H. Training-induced morphological and functional changes in skeletal muscle.  Int J Sports Med. 1982;  3 1-12
• 11 Hughes SM, Cho M, Karsch-Mizrachi I, Travis M, Silberstein L, Leinwand LA, Blau HM. Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.  Dev Biol. 1993;  158 183-199
• 12 Kadi F, Charifi N, Denis C, Lexell J, Andersen JL, Schjerling P, Olsen S, Kjaer M. The behaviour of satellite cells in response to exercise: what have we learned from human studies?.  Pflugers Arch. 2005;  451 319-327
• 13 Kadi F, Charifi N, Denis C, Lexell J. Satellite cells and myonuclei in young and elderly men and women.  Muscle Nerve. 2004;  29 120-127
• 14 Kadi F, Schjerling P, Andersen LL, Charifi N, Madsen JL, Christensen LR, Andersen JL. The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles.  J Physiol. 2004;  558 1005-1012
• 15 Kadi F. Adaptation of human skeletal muscle to training and anabolic steroids.  Acta Physiol Scand Suppl. 2000;  646 1-52
• 16 Kadi F, Thornell LE. Concomitant increases in myonuclear and satellite cell content in female trapezius muscle following strength training.  Histochem Cell Biol. 1999;  113 99-103
• 17 Kadi F, Hägg G, Håkansson R, Holmner S, Butler-Browne GS, Thornell LE. Structular changes in male trapezius muscle with work-related myalgia.  Acta Neuropathol. 1998;  95 352-360
• 18 Kesidis N, Metaxas TI, Vrabas IS, Stefanidis P, Vamvakoudis E, Christoulas K, Mandroukas A, Balasas D, Mandroukas K. Myosin heavy chain isoform distribution in single fibres of bodybuilders.  Eur J Appl Physiol. 2008;  103 579-583
• 19 Klitgaard H, Zhou M, Richter EA. Myosin heavy chain composition of single fibers from m. biceps brachii of male body builders.  Acta Physiol Scand. 1990;  140 175-180
• 20 Mavidis A, Vamvakoudis E, Metaxas T, Stefanidis P, Koutlianos N, Christoulas K, Karamanlis A, Mandroukas K. Morphology of deltoid muscles in elite tennis players.  J Sports Sci. 2007;  25 1501-1506
• 21 Nilsson J, Csergö S, Gullstrand L, Tveit P, Refsnes PE. Work-time profile, blood lactate concentration and rating of perceived exertion in the 1998 Greco-Roman Wrestling World Championship.  J Sports Sci. 2002;  20 939-945
• 22 Pette D, Staron RS. Myosin isoforms, muscle fiber types, and transitions.  Microsc Res Tech. 2000;  50 500-509
• 23 Saltin B, Henriksson J, Nygaard E, Andersen P, Jansson E. Fiber types and metabolic potentials of skeletal muscles in sedentary man and endurance runners.  Ann N Y Acad Sci. 1977;  301 3-29
• 24 Salviati G, Betto R, Danieli-Betto D, Zeviani M. Myofibrillar-protein isoforms and sarcoplasmic Ca⁺⁺-transport activity of single muscle human fibres.  Biochem J. 1984;  224 215-225
• 25 Schubert W, Zimmermann K, Cramer M, Starzinski-Powitz A. Lymphocyte antigen Leu-19 as a molecular marker of regeneration in human skeletal muscle.  Proc Natl Acad Sci USA. 1989;  86 307-311
• 26 Schultz E, McCormick KM. Skeletal muscle satellite cells.  Rev Physiol Biochem Pharmacol. 1994;  23 213-257
• 27 Sharratt MT, Taylor AW, Song TM. A physiological profile of elite Canadian freestyle wrestlers.  Can J Appl Sport Sci. 1986;  11 100-105
• 28 Shoepe TC, Stelzer JE, Garner DP, Widrick JJ. Functional adaptability of muscle fibers to long-term resistance exercise.  Med Sci Sports Exerc. 2003;  35 944-951
• 29 Sinha-Hikim I, Artaza J, Woodhouse L, Gonzalez-Cadavid N, Singh AB, Lee MI, Storer TW, Casaburi R, Shen R, Bhasin S. Testosterone-induced increase in muscle size in healthy young men is associated with muscle fiber hypertrophy.  Am J Physiol. 2002;  283 E154-E164
• 30 Smerdu V, Karsch-Mizrachi I, Campione M, Leinwand L, Schiaffino S. Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle.  Am J Physiol. 1994;  267 C1723-C1728
• 31 Staron RS, Karapondo DL, Kraemer WJ, Fry AC, Gordon SE, Falkel JE, Hagerman FC, Hikida RS. Skeletal muscle adaptations during early phase of heavy-resistance training in men and women.  J Appl Physiol. 1994;  76 1247-1255
• 32 Tesch PA, Karlsson J. Muscle fiber types and size in trained and untrained muscles of elite athletes.  J Appl Physiol. 1985;  59 1716-1720
• 33 Tesch PA, Larsson L. Muscle hypertrophy in bodybuilders.  Eur J Appl Physiol. 1982;  49 301-306
• 34 Tesch PA, Thorsson A, Kaiser P. Muscle capillary supply and fiber type characteristics in weight and power lifters.  J Appl Physiol. 1984;  56 35-38

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