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Cent Eur Neurosurg 2011; 72(1): 38-41
DOI: 10.1055/s-0030-1249699
Technical Note

© Georg Thieme Verlag KG Stuttgart · New York

Microsurgically Induced Pure Arterial Aneurysm Model in Rats

T. Mücke1 , F. Hölzle1 , K.-D. Wolff1 , A. Harders2 , M. Scholz2
  • 1Technische Universität München, Department of Oral and Maxillofacial Surgery, Munich, Germany
  • 2Ruhr-University Bochum, Department of Neurosurgery, Bochum, Germany
Further Information

Publication History

Publication Date:
13 December 2010 (online)

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Abstract

Introduction: The aim of the study was to develop a reliable and reproducible arterial aneurysm model for microsurgical training and further research with dimensions comparable to those encountered in aneurysms in humans.

Methods: The arterial aneurysm models were created microsurgically at the bifurcation of the abdominal aorta using a graft of the carotid artery in 20 Wistar rats.

Results: The aneurysms were created successfully and no complications occurred. The average volume of this arterial aneurysm model was 35.19±5.64 mm3. The time required to create this kind of aneurysm was 192±14.4 min. The central zone of blood inflow into the aneurysm was not affected by any thrombus formation.

Conclusion: The presented model at the aortic bifurcation in the rat is reliable and immediately available for microsurgical and technical training as well as for scientific studies on aneurysms. Since this kind of model also reproduces arterial aneurysms, basic techniques such as suturing and microtechniques needed for the dissection and repair of vessels can be taught during its creation.

Key words

arterial aneurysm - microvascular creation - animal model - experimental - rat

References

  • 1 Molyneux A, Kerr R, Stratton I. et al . International Subarachnoid Aneurysm Trial (ISAT) of neurosurgical clipping versus endovascular coiling in 2143 patients with ruptured intracranial aneurysms: a randomised trial.  Lancet. 2002;  360 1267-1274
    Google Scholar
  • 2 Niemela M, Koivisto T, Kivipelto L. et al . Microsurgical clipping of cerebral aneurysms after the ISAT Study.  Acta Neurochir Suppl. 2005;  94 3-6
    Google Scholar
  • 3 Aboud E, Al-Mefty O, Yasargil MG. New laboratory model for neurosurgical training that simulates live surgery.  J Neurosurg. 2002;  97 1367-1372
    Google Scholar
  • 4 Matsumura N, Hayashi N, Hamada H. et al . A newly designed training tool for microvascular anastomosis techniques: microvascular practice card.  Surgical Neurology. 2009;  71 616-620
    Google Scholar
  • 5 Menovsky T. A human skull cast model for training of intracranial microneurosurgical skills.  Microsurgery. 2000;  20 311-313
    Google Scholar
  • 6 Johnston SC, Zhao S, Dudley RA. et al . Treatment of unruptured cerebral aneurysms in California.  Stroke. 2001;  32 597-605
    Google Scholar
  • 7 Johansson M, Norback O, Gal G. et al . Clinical outcome after endovascular coil embolization in elderly patients with subarachnoid hemorrhage.  Neuroradiology. 2004;  46 385-391
    Google Scholar
  • 8 Strother CM, Graves VB, Rappe A. Aneurysm hemodynamics: an experimental study.  AJNR Am J Neuroradiol. 1992;  13 1089-1095
    Google Scholar
  • 9 Yasargil MG. Microsurgical Anatomy of the Basal Cisterns and Vessels of the Brain. Diagnostic Studies, General Operative Techniques and Pathological Considerations of the Intracranial Aneurysms.. In: Microneurosurgery. New York Georg Thieme Verlag; 1984: 54-128 279–303
    Google Scholar
  • 10 Marjamaa J, Tulamo R, Frosen J. et al . Occlusion of neck remnant in experimental rat aneurysms after treatment with platinum- or polyglycolic-polylactic acid-coated coils.  Surg Neurol. 2009;  71 458-465 ; discussion 465
    Google Scholar
  • 11 Mucke T, Scholz M, Kesting MR. et al . Microsurgically induced aneurysm models in rats, Part II: clipping, shrinking and micro-Doppler sonography.  Minim Invasive Neurosurg. 2008;  51 6-10
    Google Scholar
  • 12 Guglielmi G, Ji C, Massoud TF. et al . Experimental saccular aneurysms. II. A new model in swine.  Neuroradiology. 1994;  36 547-550
    Google Scholar
  • 13 Hoh BL, Rabinov JD, Pryor JC. et al . A modified technique for using elastase to create saccular aneurysms in animals that histologically and hemodynamically resemble aneurysms in human.  Acta Neurochir (Wien). 2004;  146 705-711
    Google Scholar
  • 14 Krings T, Busch C, Sellhaus B. et al . Long-term histological and scanning electron microscopy results of endovascular and operative treatments of experimentally induced aneurysms in the rabbit.  Neurosurgery. 2006;  59 911-923 ; discussion 923–914
    Google Scholar
  • 15 Krings T, Moller-Hartmann W, Hans FJ. et al . A refined method for creating saccular aneurysms in the rabbit.  Neuroradiology. 2003;  45 423-429
    Google Scholar
  • 16 Spetzger U, Reul J, Weis J. et al . Endovascular coil embolization of microsurgically produced experimental bifurcation aneurysms in rabbits.  Surg Neurol. 1998;  49 491-494
    Google Scholar
  • 17 Spetzger U, Reul J, Weis J. et al . Microsurgically produced bifurcation aneurysms in a rabbit model for endovascular coil embolization.  J Neurosurg. 1996;  85 488-495
    Google Scholar
  • 18 Kallmes DF, Helm GA, Hudson SB. et al . Histologic evaluation of platinum coil embolization in an aneurysm model in rabbits.  Radiology. 1999;  213 217-222
    Google Scholar
  • 19 Lee D, Yuki I, Murayama Y. et al . Thrombus organization and healing in the swine experimental aneurysm model. Part I. A histological and molecular analysis.  J Neurosurg. 2007;  107 94-108
    Google Scholar
  • 20 Guerreiro NE, Colli BO, Carlotti Jr CG. et al . Experimental microaneurysms in rats: I. Model for induction.  Surg Neurol. 2004;  62 406-412
    Google Scholar
  • 21 Handa H, Hashimoto N, Nagata I. et al . Saccular cerebral aneurysms in rats: a newly developed animal model of the disease.  Stroke. 1983;  14 857-866
    Google Scholar
  • 22 Kirse DJ, Flock S, Teo C. et al . Construction of a vein-pouch aneurysm at a surgically created carotid bifurcation in the rat.  Microsurgery. 1996;  17 681-689
    Google Scholar
  • 23 Thiex R, Moller-Hartmann W, Hans FJ. et al . Are the configuration and neck morphology of experimental aneurysms predictable?.  A technical approach. Neuroradiology. 2004;  46 571-576
    Google Scholar
  • 24 Scholz M, Mucke T, During M. et al . Microsurgically induced aneurysm models in rats, part I: techniques and histological examination.  Minim Invasive Neurosurg. 2008;  51 76-82
    Google Scholar
  • 25 Abruzzo T, Shengelaia GG, Dawson 3rd RC. et al . Histologic and morphologic comparison of experimental aneurysms with human intracranial aneurysms.  AJNR Am J Neuroradiol. 1998;  19 1309-1314
    Google Scholar
  • 26 Scholz M, Mucke T, Holzle F. et al . A program of microsurgical training for young medical students: are younger students better?.  Microsurgery. 2006;  26 450-455
    Google Scholar
  • 27 Yasargil MG. Clinical Considerations, Surgery of the Intracranial Aneurysms and Results.. In: Microneurosurgery. New York Georg Thieme Verlag; 1984: 347-348
    Google Scholar
  • 28 Sugiu K, Martin JB, Jean B. et al . Artificial cerebral aneurysm model for medical testing, training, and research.  Neurol Med Chir (Tokyo). 2003;  43 69-72 ; discussion 73
    Google Scholar
  • 29 Ferguson GG. Physical factors in the initiation, growth, and rupture of human intracranial saccular aneurysms.  J Neurosurg. 1972;  37 666-677
    Google Scholar

Correspondence

T. MückeMD 

Technische Universität

München

Department of Oral and

Maxillofacial Surgery

Ismaninger Straße 22

81675 Munich

Germany

Phone: +49/89/140 29 21

Fax: +49/89/140 49 93

Email: th.mucke@gmx.de