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Planta Med 2010; 76(14): 1570-1575
DOI: 10.1055/s-0030-1249778
Pharmacology
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Constituents of Cinnamon Inhibit Bacterial Acetyl CoA Carboxylase

Glen Meades1  Jr. , Rachel L. Henken2 , Grover L. Waldrop1 , Md. Mukhlesur Rahman3 , S. Douglass Gilman2 , Guy P. P. Kamatou4 , Alvaro M. Viljoen4 , Simon Gibbons3
  • 1Division of Biochemistry and Molecular Biology, Louisiana State University, Baton Rouge, Louisiana, USA
  • 2Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana, USA
  • 3Department of Pharmaceutical and Biological Chemistry, The School of Pharmacy, University of London, London, UK
  • 4Department of Pharmaceutical Sciences, Faculty of Science, Tshwane University of Technology, Pretoria, South Africa
Weitere Informationen

Publikationsverlauf

received Dec. 7, 2009 revised February 22, 2010

accepted March 4, 2010

Publikationsdatum:
08. April 2010 (online)

Auch verfügbar auf
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Abstract

Cinnamon bark (Cinnamomum zeylanicum) is used extensively as an antimicrobial material and currently is being increasingly used in Europe by people with type II diabetes to control their glucose levels. In this paper we describe the action of cinnamon oil, its major component, trans-cinnamaldehyde, and an analogue, 4-hydroxy-3-methoxy-trans-cinnamaldehyde against bacterial acetyl-CoA carboxylase in an attempt to elucidate the mechanism of action of this well-known antimicrobial material. These natural products inhibited the carboxyltransferase component of Escherichia coli acetyl-CoA carboxylase but had no effect on the activity of the biotin carboxylase component. The inhibition patterns indicated that these products bound to the biotin binding site of carboxyltransferase with trans-cinnamaldehyde having a Ki value of 3.8 ± 0.6 mM. The inhibition of carboxyltransferase by 4-hydroxy-3-methoxy-trans-cinnamaldehyde was analyzed with a new assay for this enzyme based on capillary electrophoresis. These results explain, in part, the antibacterial activity of this well-known antimicrobial material.

Key words

Cinnamomum zeylanicum - Lauraceae - trans‐cinnamaldehyde - acetyl‐CoA carboxylase - carboxyltransferase

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Simon Gibbons

Department of Pharmaceutical and Biological Chemistry
The School of Pharmacy
University of London

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