Antimicrobial Activity of Submerged Cultures of Chilean Basidiomycetes

Abstract

This study is part of a screening program aimed at searching for bioactive metabolites from Chilean basidiomycetes. Submerged cultivation of fungal mycelia in liquid media was evaluated for antimicrobial activity. A total of 148 strains were obtained in vitro. The extracts produced from submerged cultures were evaluated against bacteria and fungi. In the primary antimicrobial assay, approximately 60 % of the extracts presented positive biological activity. The highest frequencies of active strains were from the orders Agaricales (31.0 %), Polyporales (20.6 %), Sterales (18.3 %), Boletales (11.4 %), and Cortinariales (9.1 %). Antifungal activity was more pronounced than antibacterial activity. Twelve extracts that exhibited strong antimicrobial activity showed minimum inhibitory concentration (MIC) values of 50 µL/mL against Bacillus brevis and 25 ∼ 50 µL/mL against Penicillium notatum and Paecilomyces variotii. The biological activity of some strains did not vary considerably, regardless of the substrate or collection site whereas, for others, it showed marked variations. Differences in antimicrobial activities observed in the different fungal genera suggested that the ability to produce bioactive compounds is not homogenously distributed among basidiomycetes. The information obtained from this study reveals that Chilean basidiomycetes are able to generate small and/or large variations in the normal pathway of compounds production. Thus, it is necessary to evaluate this biological and chemical wealth, which could be an unsuspected reservoir of new and potentially useful molecules.

Key words

antibacterial - antifungal - basidiomycetes - fungi - biological activity

References

• 1 Brizuela M A, García L, Pérez L, Mansur M. Basidiomicetos: nueva fuente de metabolitos secundarios.  Rev Iberoam Micol. 1998;  15 69-74
• 2 Lorenzen K, Anke T. Basidiomycetes as a source for new bioactive natural products.  Curr Org Chem. 1998;  2 329-364
• 3 Abraham W R. Bioactive sesquiterpenes produced by fungi: are they useful for humans as well?.  Curr Med Chem. 2001;  8 583-606
• 4 Keller C, Maillard M, Keller J, Hostettmann K. Screening of European fungi for antibacterial, antifungal, larvicidal, molluscicidal, antioxidant and free-radical scavenging activities and subsequent isolation of bioactive compounds.  Pharm Biol. 2002;  40 518-525
• 5 Garrido N. Index Agaricalium chilesium. Bibliotheca Mycologica, Vol. 99. Vaduz; Cramer 1985: 1-567
• 6 Garrido N. Agaricales s.l. und ihre Mykorrhizen in den Nothofagus-Wäldern Mittelchiles. Cramer J Bibliotheca Mycologica, Vol. 120. Springer; Berlin, Stuttgart 1988: 1-528
• 7 Hoeneisen M, Garrido N, Silva M. Antibacterial activity of Basidiomycetes associated with Nothofagus woods.  Acta Microbiol. 1993;  1 47-52
• 8 Schmeda G, Villaseñor M, Lozoya X, Puebla A. Immunomodulatory activity of Chilean Cyttaria species in mice with L5178Y lymphoma.  J Ethnopharmacol. 2001;  77 253-257
• 9 Clericuzio M, Piovano M, Chamy M, Garbarino J, Milanesio M, Viterbo D, Vidari G, Vita Finzi P. Structural characterisation of metabolites from Pholiota spumosa (Basidiomycetes).  Croat Chem Acta. 2004;  77 605-611
• 10 Eriksson J, Ryvarden L. The Corticiaceae of North Europe.  Fungiflora. 1976;  4 549-886
• 11 Horak E, Moser M. Fungi Austroamericani. XII Studien zur Gattung Thaxterogaster Sing.  Nova Hedwigia. 1965;  10 211-241
• 12 Rajchenberg M. El género Coriolus (Polyporaceae) en la República Argentina.  Bol Soc Argent Bot. 1982;  21 17-57
• 13 Rojas de la Parra V, Mierau V, Anke T, Sterner O. Niveulone, a heterocyclic spiro terpenoid from the ascomycete Dasyscyphus niveus.  J Antibiot. 2006;  59 57-60
• 14 Silberborth S, Erkel G, Anke T, Sterner O. The irpexans, a new group of biologically active metabolites produced by the basidiomycete Irpex sp. 93028.  J Antibiot. 2000;  53 1137-1144
• 15 Anke H, Bergendorff O, Sterner O. Assays of the biological activities of guaiane sesquiterpenoids isolated from the fruit bodies of edible Lactarius species.  Food Chem Toxicol. 1989;  27 393-397
• 16 Anke T, Anke H, Erkel G. New products from higher fungi.  Screening. 2001;  2 4-53
• 17 Thines E, Anke H, Sterner O. Trichoflectin, a bioactive azaphilone from the ascomycete Trichopezizella nidulus.  J Nat Prod. 1998;  61 306-308
• 18 Mierau V, Anke T, Sterner O. Dacrymenone and VM 3298-2- new antibiotics with antibacterial and antifungal activity.  Z Naturforsch. 2003;  58c 541-546
• 19 Vollekova A, Kostalova D, Sochorova R. Isoquinoline alkaloids from Mahonia aquifolium stem bark is active against Malassezia sp.  Folia Microbiol. 2001;  46 107-111
• 20 Srinivasan D, Nathan S, Suresh T, Perumalsamy P. Antimicrobial activities of certain Indian medicinal plants in folkloric medicine.  J Ethnopharmacol. 2001;  74 217-220
• 21 Suay I, Arenal F, Asensio F J, Basilio A, Cabello M A, Díez M T, García J B, Val A G, Gorrochategui J, Hernández P, Peláez F, Vicente M F. Screening of basidiomycetes for antimicrobial activities.  Anton Leeuw INTJG. 2000;  78 129-139
• 22 Rosa L H, Machado K, Jacob C, Capelari M, Rosa C, Zani C. Screening of Brazilian basidiomycetes for antimicrobial activity.  Mem Inst Oswaldo Cruz. 2003;  98 967-974
• 23 Wildman H. Influence of habitation the physiological and metabolic diversity of fungi.  Can J Bot. 1995;  73 s907-s916
• 24 Talbot N, Vincent P, Wildman H. The influence of genotype and environment on the physiological and metabolic diversity of Fusarium compactum.  Fungal Genet Biol. 1996;  20 254-267
• 25 Larsen T O, Smedsgaard J, Nielsen K F, Hansen M E, Frisvad J C. Phenotypic taxonomy and metabolite profiling in microbial drug discovery.  Nat Prod Rep. 2005;  22 672-695
• 26 Frisvad J C, Larsen T O, de Vries R, Meijer M, Houbraken J, Cabañes F J, Ehrlich K, Samson R A. Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins.  Stud Mycol. 2007;  59 31-37