Diterpene Alkaloids from Aconitum anthora and Assessment of the hERG-Inhibiting Ability of Aconitum Alkaloids

Abstract

A new norditerpene alkaloid, 10-hydroxy-8-O-methyltalatizamine (1), was isolated from the whole plant of Aconitum anthora L. besides the known isotalatizidine (2) and hetisinone (3). The structures were determined by means of HR-ESI‐MS, 1D and 2D NMR spectroscopy, including ¹H-¹H COSY, NOESY, HSQC and HMBC experiments, resulting in complete ¹H and ¹³C chemical shift assignments for 1–3, and revision of some earlier ¹³C‐NMR data. The effects of the isolated compounds, together with twenty-one other Aconitum alkaloids with different skeletal types and substitution patterns, on hERG channels were studied by the whole-cell patch clamp technique, using the QPatch-16 automated patch clamp system. At 10 µM, aconitine, 14-benzoylaconine 8-O-palmitate, songoramine, gigactonine and neolinine demonstrated significant hERG K⁺ channel inhibition; all other compounds exerted only low (6–21 %) inhibitory activity.

Key words

Aconitum anthora - Ranunculaceae - aconitine - diterpene alkaloids - hERG - potassium channels

References

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