Abstract
Sabia parviflora Wall. ex Roxb. is a traditional herb widely used by Chinese people, especially by the Buyi ethnic group which resides in Guizhou and Yunnan provinces. According to the Chinese Ethnic Pharmacopeia, the species is commonly used for soothing the liver and for the treatment of icteric hepatitis, hemostasis, and inflammation. However, due to the similar morphological characters of Sabia species and higher market demands, there are many substitutes and adulterants of S. parviflora. In this study, the differential identification of 6 Sabia species and 7 adulterants were investigated through DNA sequence analysis of three candidate DNA barcodes (trnH-psbA, rbcL‐a, matK). Based on sequence alignments, we concluded that not only the trnH-psbA spacer sequence can distinguish S. parviflora from other Sabia species, but the matK + rbcL‐a sequences also can differentiate it from the substitutes and adulterants. The classification tree of all samples based on rbcL‐a sequences indicated that the rbcL region can identify samples into a family/genus level. Our results suggest that the three candidate barcodes can be used for the identification of S. parviflora and to distinguish it from common substitutes or adulterants.
Key words
Sabia parviflora
- Sabiaceae - DNA barcoding - ethnomedicinal plant - authentication
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Prof. Dr. Chun-lin Long
Key Laboratory of Economic Plants and Biotechnology
Kunming Institute of Botany
Chinese Academy of Sciences
132 Lanhei Road
Kunming 650204
Yunnan
People's Republic of China
Telefon: +86 87 15 22 32 33
Fax: +86 87 15 22 32 33
eMail: long@mail.kib.ac.cn
