New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Günther Heubl

doi:10.1055/s-0030-1250519

Planta Medica, Inhaltsverzeichnis

Planta Med 2010; 76(17): 1963-1974
DOI: 10.1055/s-0030-1250519

Traditional Chinese Medicine

Reviews
© Georg Thieme Verlag KG Stuttgart · New York

New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques

Günther Heubl¹

¹Biology Department I – Systematic Botany, Ludwig-Maximilians University Munich, Munich, Germany

Abstract

DNA technology provides a powerful tool to complement chemical analyses for authentication of Chinese medicinal plants and to ensure that herbal materials are not contaminated with ineffective or potentially harmful substitutes or adulterants. In the last two decades molecular biotechnology has provided sophisticated molecular techniques for authentication of botanical materials at the DNA level. This review provides an account of the most commonly used DNA-based technologies (RAPD, RFLP, ARMS, CAPS, AFLP, DAF, ISSR, SSR, sequencing, hybridization and microarrays) including suitable examples of Chinese medical plants. A critical evaluation of all methods is presented concerning sensitivity, reliability, reproducibility, and running costs. Recent achievements in the field of DNA barcoding and DNA chip technology that offer great potentials for screening of DNA and emerging new developments for future identification of species are briefly outlined.

Key words

authentication - species identification - Chinese medicinal plants - standardization - molecular markers - DNA technologies

Volltext

Referenzen

References

1 Lopez-Pujol J L, Zahng F M, Song G E. Plant biodiversity in China: richly varied, endangered, and in need of conservation. Biodivers Conserv. 2006; 15 3983-4026
2 Qian H, Ricklefs R E. A comparison of the taxonomic richness of vascular plants in China and the United States. Am Nat. 1999; 154 160-181
3 Zhong Y D. Big Chinese Herb Dictionary. Shanghai Kexue Jishu Chu Ban Shi. Shanghai; Shanghai Science and Technology Publishing Co. 1977
4 Lee H H, Itokawa H, Kozuka M. Asian herbal products: The basis for development of high-quality dietary supplements and new medicines. Shi J, Ho CT, Shahidi F Asian Functional Foods. Boca Raton; Pub CRC Press, Taylor & Francis Group 2005
5 Zhao Z Z, Hu Y, Liang Z T, Yuen P S J, Jiang Z H, Leung K S Y. Authentication is fundamental for standardization of Chinese medicines. Planta Med. 2006; 72 865-874
6 Zhao Z Z, Li T Y S. Easily confused Chinese medicines in Hong Kong (English edition). Hong Kong; Chinese Medicine Merchants Association Ltd. 2007
7 Chan T Y, Critchley J A. Usage and adverse effects of Chinese herbal medicines. Hum Exp Toxicol. 1996; 15 5-12
8 Gertner E, Marshall P S, Filandrinos D, Potek A S, Smith T M. Complications resulting from the use of Chinese herbal medications containing undeclared prescription drugs. Arthritis Rheum. 1995; 38 614-617
9 But P P, Tomlinson B, Cheung K O, Yong S P, Szeto M L, Lee C K. Adulterants of herbal products can cause poisoning. Br Med J. 1996; 313 117
10 Chen J, Chen L, An Z, Shi S, Zhan Y. Non-technical causes of fakes existing in Chinese medicinal material markets. Zhongyaocai. 2002; 25 516-519
11 Zhao Z, Hu Y, Liang Z, Yuen J, Jiang Z. Leung KSY. Authentication is fundamental for standardization of Chinese medicines. Planta Med. 2006; 72 865-874
12 Huang H H, Yen D H T, Wu M L, Deng J F, Huang C I, Lee C H. Acute Erycibe henryi Prain (“Ting Kung Teng”) poisoning. Clin Toxicol. 2006; 44 71-75
13 Sun S Q, Zhou Q, Liu J, Huang H. Study on the identification of standard and false BanXia by two-dimensional infrared correlation spectroscopy. Spectrosc Spectr Anal. 2004; 24 427-430
14 Zhang W H, Shen Z J. Comparison on macroscopic characteristics of clinical curative effect of Banxia and Shuibanxia. J Nanjing Univ Tradit Chin Med. 1995; 11 32-33
15 Zhao Z Z, Li T Y S. Hong Kong Commonly Confused Chinese Medicines. Hong Kong; Chinese Medicine Merchants Association Ltd. 2004
16 Society of Japanese Pharmacopoeia .Japanese Pharmacopoeia (English version), 14th edition. Tokyo; Junkudo Book Shop 2001
17 Korean Food and Drug Administration .Korean Herbal Pharmacopoeia, 8th edition. Seoul; Ministry of Health Family Welfare of South Korea 2002
18 State Pharmacopoeia Committee .Pharmacopoeia of China (2005 edition). Beijing; Chemical lndustry Publisher 2005
19 Siow Y L, Gong Y, Au-Yeung K K, Woo C W, Choy P C. Emerging issues in traditional Chinese medicine. Can J Physiol Pharmacol. 2005; 83 321-334
20 Chan K. Some aspects of toxic contaminants in herbal medicines. Chemosphere. 2003; 52 1361-1371
21 Shaw P C, Ngan F N, But P P H, Wang J. Molecular markers in Chinese medicinal materials. Shaw PC, But PPH Authentication of Chinese medicinal material by DNA technology. Singapore; World Scientific Publishing 2002
22 Zhang Y B, Shaw P C, Sze C W, Wang Z T, Tong Y. Molecular authentication of Chinese herbal materials. J Food Drug Anal. 2007; 15 1-9
23 Sucher J N, Carles M C. Genome-based approaches to the authentication of medicinal plants. Planta Med. 2008; 74 603-623
24 Shaw P C, Wong K K L, Chan A W K, Wong W C, But P P H. Patent applications for using DNA technologies to authenticate medicinal herbal material. J Chin Med. 2009; 4 1-11
25 Yip P Y, Chau C F, Mak C Y, Kwan H S. DNA methods for identification of Chinese medicinal materials. J Chin Med. 2007; 2 1-19
26 Kaplan J, Chavan P, Warude D, Patwardhan B. Molecular markers in herbal drug technology. Curr Sci. 2004; 87 159-165
27 Pereira F, Carneiro J, Amorim A. Identification of species with DNA-based technology: current progress and challenges. Recent Pat DNA Gene Seq. 2008; 2 187-200
28 Ratnasingham S, Hebert P D N. BOLD: the barcode of life data system (http://www.barcodinglife.org). Mol Ecol Notes. 2007; 7 355-364
29 Chase M W, Salamin N, Wilkinson M, Dunwell J M, Kesanakurthi R P, Haidar N, Savolainen V. Land plants and DNA barcodes: short-term and long-term goals. Philos Trans R Soc Lond Ser B Biol Sci. 2005; 360 1889-1895
30 Trau D, Lee T M, Lao A I, Lenigk R, Hsing I M, Ip N Y, Carles M C, Sucher N J. Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray. Anal Chem. 2002; 74 3168-3173
31 Schena M, Heller R A, Theriault T P, Konrad K, Lachenmeier E, Davis R W. Microarrays: biotechnology's discovery platform for functional genomics. Trends Biotechnol. 1998; 16 301-316
32 Lashermes P, Combes M C, Cros J. Use of non-radioactive digoxigenin-labelled DNA probes for RFLP anlyses in coffee. Techique et utilisations des marqueurs moleculaires. 1994; 72 29-31
33 Yamazaki M, Sato A, Saito K, Murakoshi I. Molecular phylogeny based on RFLP and its relation with alkaloid patterns in Lupinus plants. Biol Pharm Bull. 1993; 16 1182-1184
34 Trifi-Farah N, Marrakchi M. Hedysarum phylogeny mediated by RFLP analysis of nuclear ribosomal DNA. Genet Resour Crop Evol. 2001; 48 339-345
35 Mori N, Moriguchi T, Nakamura C. RFLP analysis of nuclear DNA for study of phylogeny and domestication of tetraploid wheat. Genes Genet Syst. 1997; 72 153-161
36 Gawel N J, Jarret R L, Whittemore A P. Restriction fragment length polymorphism (RFLP)-based phylogenetic analysis of Musa. Theor Appl Genet. 1992; 84 286-290
37 Li T, Wang J, Lu Z. Accurate identification of closely related Dendrobium species with multiple species-specific gDNA probes. J Biochem Biophys Methods. 2005; 62 111-123
38 Tsoi P Y, Woo H S, Wong M S, Chen S L, Fong W F, Xiao P G, Yang M S. Genotyping and species identification of Fritillaria by DNA chips. Yaoxue Xuebao. 2003; 38 185-190
39 Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990; 18 7213-7218
40 Munthali M, Ford-Lloyd B V, Newbury H J. The random amplification of polymorphic DNA for fingerprinting plants. PCR Methods Appl. 1992; 1 274-276
41 Kersten T, Daniel C, König G M, Knöß W. The potential of PCR-related methods to identify medicinal plants in herbal medicinal products. Planta Med. 2007; 73 256
42 Yamasaki M, Sato A, Shimomura K, Saito K, Murakoshi I. Genetic relationships among Glycyrrhiza plants determined by RADP and RFLP analyses. Biol Pharm Bull. 1994; 17 1529-1531
43 Kohjyouma M, Nakajima S, Namera A, Shimizu R, Mizukami H, Kohda H. Random amplified polymorphic DNA analysis and variation of essential oil components of Atractylodes plants. Biol Pharm Bull. 1997; 20 502-506
44 Chen K T, Su Y C, Lin J G, Hsin L H, Su Y P, Su C H, Li S Y, Cheng J H, Mao S J. Identification of Atractylodes plants in Chinese herbs and formulations by random amplified polymorphic DNA. Acta Pharmacol Sin. 2001; 22 493-497
45 Cheng K T, Su B, Chen C T, Lin C C. RAPD analysis of Astragalus medicines marketed in Taiwan. Am J Chin Med. 2000; 28 273-278
46 Wang P, Huang F, Zhou L, Cao L, Liang S, Xu H, Liu J. Analysis of Amomun villosum species and some adulterants of Zingiberaceae by RAPD. Zhong Yao Cai. 2000; 23 71-74
47 Hosokawa K, Minami M, Kawahara K, Nakamura I, Shibata T. Discrimination among three species of medicinal Scutellaria plants using RAPD markers. Planta Med. 2000; 66 270-272
48 Cheung K S, Kwan H S, But P P H, Shaw P C. Pharmacognostical identification of American and Oriental ginseng roots by genomic fingerprinting using arbitrarily primed polymerase chain reaction (AC-PCR). J Ethnopharmacol. 1994; 42 67-69
49 Shim Y H, Choi J H, Park C D, Lim C J, Cho J H, Kim H J. Molecular differentiation of Panax species by RAPD analysis. Arch Pharm Res. 2003; 26 601-605
50 Cui X M, Lo C K, Yip K L, Dong T T, Tsim K W. Authentication of Panax notoginseng by 5S-rRNA spacer domain and random amplified polymorphic DNA (RAPD) analysis. Planta Med. 2003; 69 584-586
51 Lim W, Mudge K W, Weston L. Utilization of RAPD markers to assess genetic diversity of wild populations of North American ginseng (Panax quinquefolium). Planta Med. 2007; 73 71-76
52 Cole C T, Kuchenreuther M A. Molecular markers reveal little genetic differentiation among Aconitum noveboracense and A. columbianum (Ranunculaceae) populations. Am J Bot. 2001; 88 337-347
53 Fan X X, Shen L, Zhang X, Chen X Y, Fu C X. Assessing genetic diversity of Ginkgo biloba L. (Ginkgoaceae) populations from China by RAPD markers. Biochem Genet. 2004; 42 269-278
54 Cheng K T, Fu L C, Wang C S, Hsu F L, Tsay H S. Identification of Anoectochilus formosanus and Anoectochilus koshunensis species with RAPD markers. Planta Med. 1998; 64 46-49
55 Zhang K Y B, Leung H W, Yeung H W, Wong R N S. Differentiation of Lycium barbarum from its related Lycium species using random amplified polymorphic DNA. Planta Med. 2001; 67 379-381
56 Watanabe A, Araki S, Kobari S, Sudo H, Tsuchida T, Uno T, Kosaka N, Shimomura K, Yamazaki M, Saito K. In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants. Plant Cell Rep. 1998; 18 187-192
57 Liang Z T, Qin M J, Wang Z T, Huang Y, Wang N H. Identification of Bupleurum L. plants by RAPD technology. Zhongcaoyào. 2002; 33 1117-1119
58 Zhang M, Huang H R, Liao S M, Gao J Y. Cluster analysis of Dendrobium by RAPD and design of specific primer for Dendrobium candidum. Zhongguo Zhong Yao Za Zhi. 2001; 26 442-447
59 Guo B L, Wu M, Si J P, Li J S, Xiao P G. Research on DNA molecular marker of Magnolia officinalis Rehd. et Wils. RAPD study on certified species. Yao Xue Xue Bao. 2001; 36 386-389
60 Huang L, Wang M, Zhou C, Li N, He X, Yang B. Problems and solutions in the use of RAPD to the identification of the Chinese drugs “xi-xin” (Herba Asari) and its substitutes. Yao Xue Xue Bao. 1998; 33 778-784
61 Lu C, Zhang W, Peng X, Gu G, Chen M, Tang Z. Development of randomly amplified polymorphic DNA-sequence characterized amplified region marker for identification of Apocynum venetum LINN. from A. pictum SCHRENK. Biol Pharm Bull. 2010; 33 522-526
62 Li Y, Ding W L. Genetic diversity assessment of Trollius accessions in China by RAPD markers. Biochem Genet. 2010; 48 34-43
63 Dnyaneshwar W, Preeti C, Kalpana J, Bhushan P. Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica L. Biol Pharm Bull. 2006; 29 2313-2316
64 Zhang R, Zhang B, Ye H. Identification of the Chinese herbs of Indigoferae L. by RAPD analysis. Hongguo Zhong Yao Za Zhi. 1997; 22 72-73
65 Cheng K T, Chang H C, Su C H, Hsu F L. Identification of dried rhizomes of Coptis species using random amplified polymorphic DNA. Bot Bull Acad Sin. 1997; 38 241-244
66 Zhang Y B, Ngan F N, Wang Z T, Wang J, But P P H, Shaw P C. Differentiation of Codonopsis pilosula using random amplified polymorphic DNA. Planta Med. 1999; 65 57-60
67 Cao H, But P P H, Shaw P C. Identification of Herba Taraxaci and its adulterants in Hong Kong market by DNA fingerprinting with random primed PCR. Chin J Chin Mater Med. 1997; 22 197-200
68 Cao H, But P P H, Shaw P C. Authentication of the Chinese drug “ku-di-dan” (Herba Elephantopi) and its substitutes using random-primed polymerase chain reaction (PCR). Acta Pharm Sin. 1996; 31 543-553
69 Cheng J L, Huang L Q, Shao A J, Lin S F. RAPD analysis on different varieties of Rehmannia glutinosa. Zhongguo Zhongyao Zazhi. 2002; 27 505-508
70 Caetano-Anollés G, Bassam B J, Gresshoff P M. DNA amplification fingerprinting: a strategy for genome analysis. Plant Mol Biol Rep. 1991; 9 294-307
71 Caetano-Anollés G, Bassam B J, Gresshoff P M. DNA amplification using very short arbitrary oligonucleotide primers. Biotechnology. 1991; 9 553-557
72 Chawla H S. Introduction to Plant Biotechnology, 2nd edition. New Hampshire; Science Publishers 2002
73 Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 1994; 20 176-183
74 Bornet B, Branchard M. Nonanchored inter simple sequence repeat (ISSR) markers: reproducible and specific tools for genome fingerprinting. Plant Mol Biol Rep. 2001; 19 209-215
75 Shen J, Ding X Y, Ding G, Liu D Y, Tang F, He J. Studies on population difference of Dendrobium officinale II establishment and optimization of the method of ISSR fingerprinting marker. Zhongguo Zhong Yao Za Zhi. 2006; 31 291-294
76 Shi H M, Wang J, Wang M Y, Tu P F, Li X B. Identification of Cistanche species by chemical and inter-simple sequence repeat fingerprinting. Biol Pharm Bull. 2009; 32 142-146
77 Li K, Wu W, Zheng Y, Dai Y, Xiang L, Liao K. Genetic diversity of Fritillaria from Sichuan province based on ISSR. Zhongguo Zhong Yao Za Zhi. 2009; 34 2149-2154
78 Song Z, Li X, Wang H, Wang J. Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica. 2010; 138 241-249
79 Hu Y, Zhang Q, Xin H, Qin L P, Lu B R, Rahman K, Zheng H. Association between chemical and genetic variation of Vitex rotundifolia populations from different locations in China: its implication for quality control of medicinal plants. Biomed Chromatogr. 2007; 21 967-975
80 Kojoma M, Iida O, Makino Y, Sekita S, Satake M. DNA fingerprinting of Cannabis sativa using inter-simple sequence repeat (ISSR) amplification. Planta Med. 2002; 68 60-63
81 Xia T, Chen S, Chen S, Zhang D, Zhang D, Gao Q, Ge X J. ISSR analysis of genetic diversity of the Qinghai-Tibet plateau endemic Rhodiola chrysanthemifolia (Crassulaceae). Biochem Syst Ecol. 2007; 35 209-214
82 Wu W, Zheng Y L, Chen L, Wei Y M, Yang R W, Yan Z H. Evaluation of genetic relationships in the genus Houttuynia Thunb. In China based on RAPD and ISSR markers. Biochem Syst Ecol. 2005; 33 1141-1157
83 Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995; 23 4407-4414
84 Vos P, Kuiper M. AFLP Analysis. Caetano-Anollés G, Gresshoff PM DNA markers: protocols, applications,and overviews. New York; Wiley-Liss 1997: 115-132
85 Blears M, De Grandis S, Lee H, Trevors J. Amplified fragment length polymorphism (AFLP): a review of the procedure and its applications. J Indian Microbiol Biotechnol. 1998; 21 99-114
86 Mueller U G, Wolfenbarger L. AFLP genotyping and fingerprinting. Trends Ecol Evol. 1999; 14 389-394
87 Choi Y E, Ahn C H, Kim B B, Yoon E S. Development of species specific AFLP-derived SCAR marker for authentication of Panax japonicus C. A. MEYER. Biol Pharm Bull. 2008; 31 135-138
88 Ha W Y, Shaw P C, Liu J, Yau F C, Wang J. Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD). J Agric Food Chem. 2002; 50 1871-1875
89 Zerega N J C, Mori S, Lindqvist C, Zheng Q, Motley T J. Using amplified fragment length polymorphisms (AFLP) to identify black cohosh (Actaea racemosa). Econ Bot. 2002; 56 154-164
90 Passinho-Soares H, Felix D, Kaplan M A, Margis-Pinheiro M, Margis R. Authentication of medicinal plant botanical identity by amplified fragmented length polymorphism dominant DNA marker: inferences from the Plectranthus genus. Planta Med. 2006; 72 929-931
91 Loh J P, Kiew R, Kee A, Gan L H, Gan Y Y. Amplified fragment length polymorphism (AFLP) provides molecular markers for the identification of Caladium bicolor cultivars. Ann Bot (Lond). 1999; 84 155-161
92 Datwyler S L, Weiblen G D. Genetic variation in hemp and marijuana (Cannabis sativa L.) according to amplified fragment length polymorphisms. J Forensic Sci. 2006; 51 371-375
93 Qi J J, Li X E, Song J, Eneji A E, Ma X. Genetic Relationships among Rehmannia glutinosa cultivars and varieties. Planta Med. 2008; 74 1846-1852
94 Weising K, Nybom H, Wolff K, Meyer W. DNA Fingerprinting in Plants and Fungi. Boca Raton; CRC Press 1995
95 Soumaya R, Dakhlaoui-Dkhil S, Salem A O M, Zehdi-Azouzi S, Rhouma A, Marrakchi M, Trifi M. Genetic diversity and phylogenic relationships in date-palms (Phoenix dactylifera L.) as assessed by random amplified microsatellite polymorphism markers (RAMPOs). Sci Hortic. 2008; 117 53-57
96 Chatti K, Saddoud O, Salhi Hannachi A, Mars M, Marrakchi M, Trifi M. Analysis of genetic diversity and relationships in a Tunisian Fig (Ficus carica) germplasm collection by random amplified microsatellite polymorphisms. J Integr Plant Biol. 2007; 49 386-391
97 Baldwin B G, Sanderson M J, Porter J M, Wojciechowski M F, Campbell C S, Donoghue M J. The ITS region of nuclear ribosomal DNA: a valuable source of evidence on angiosperm phylogeny. Ann Mo Bot Gard. 1995; 82 247-277
98 Alvarez I, Wendel J F. Ribosomal ITS sequences and plant phylogenetic inference. Mol Phylogenet Evol. 2003; 29 417-434
99 Chen S, Yao H, Han J, Liu C, Song J, Shi L, Zhu Y, Ma X, Gao T, Pang X, Luo K, Li Y, Li X, Jia X, Lin Y, Leon C. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS One. 2010; 5 1-8
100 Chiou S J, Yen J H, Fang C L, Chen H L, Lin T Y. Authentication of medicinal herbs using PCR-Amplified ITS2 with specific primers. Planta Med. 2007; 73 1421-1426
101 Ngan F, Shaw P, But P, Wang J. Molecular authentication of Panax species. Phytochemistry. 1999; 50 787-791
102 Kim O T, Bang K, In D S, Lee J W, Kim Y C, Shin Y S, Hyun D Y, Lee S S, Cha S W, Seong N S. Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences. Plant Biotechnol Rep. 2007; 1 163-167
103 Kelly L M. Phylogenetic relationships in Asarum (Aristolochiaceae) based on morphology and ITS sequences. Am J Bot. 1998; 85 1454-1467
104 Liu C S, Bai G B, Yan Y N. Studies on the botanical sources and DNA molecular identification of Herba Asari based on ITS sequence. Zhongguo Zhong Yao Za Zhi. 2005; 30 329-332
105 Yamaji H, Fukuda T, Yokoyama J, Pak J-H, Zhou C, Yang C S, Kondo K, Morota T, Takeda S, Sasaki H, Maki M. Reticulate evolution and phylogeography in Asarum sect. Asiasarum (Aristolochiaceae) documented in internal transcribed spacer sequences (ITS) of nuclear ribosomal DNA. Mol Phylogenet Evol. 2007; 44 863-884
106 Dong T T, Ma X Q, Clarke C, Song Z H, Ji Z N, Lo C K, Tsim K W. Phylogeny of Astragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. J Agric Food Chem. 2003; 51 6709-6714
107 Yip P Y, Kwan H S. Molecular identification of Astragalus membranaceus at the species and locality levels. J Ethnopharmacol. 2006; 106 222-229
108 Xu H, Wang Z, Ding X, Zhou K, Xu L. Differentiation of Dendrobium species used as “Huangcao Shihu” by rDNA ITS sequence analysis. Planta Med. 2006; 72 89-92
109 Ding X, Xu L, Wang Z, Zhou K, Xu H, Wang Y. Authentication of stems of Dendrobium officinale by rDNA ITS region sequences. Planta Med. 2002; 68 191-192
110 Lau D T, Shaw P C, Wang J, But P P. Authentication of medicinal Dendrobium species by the internal transcribed spacer of ribosomal DNA. Planta Med. 2001; 67 456-460
111 Zhang Y B, Wang J, Wang Z T, But P P, Shaw P C. DNA microarray for identification of the herb of Dendrobium species from Chinese medicinal formulations. Planta Med. 2003; 69 1172-1174
112 Wang C Z, Li P, Ding J Y, Jin G Q, Yuan C S. Identification of Fritillaria pallidiflora using diagnostic PCR and PCR-RFLP based on nuclear ribosomal DNA internal transcribed spacer sequences. Planta Med. 2005; 71 384-386
113 Yang Z Y, Chao Z, Huo K K, Wu B Y, Pan S L. Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species. Nan Fang Yi Ke Da Xue Xue Bao. 2006; 26 1593-1595
114 Luo Y M, Zhang W M, Ding X Y, Shen J, Bao S L, Chu B H, Mao S G. SNP marker and allele-specific diagnostic PCR for authenticating herbs of Perilla. Acta Pharm Sin. 2006; 41 840-845
115 Lee S K, Li P T, Lau D T, Yung P P, Kong R Y, Fong W F. Phylogeny of medicinal Phyllanthus species in China based on nuclear ITS and chloroplast atpB-rbcL sequences and multiplex PCR detection assay analysis. Planta Med. 2006; 72 721-726
116 Albach D C, Li H Q, Zhao N, Jensen S R. Molecular systematics and phytochemistry of Rehmannia (Scrophulariaceae). Biochem Syst Ecol. 2007; 35 293-300
117 Wang H, Wang Q. Analysis of rDNA ITS sequences of Radix et Rhizoma Salviae miltiorrhizae and plants of Salvia L. Chin Tradit Herb Drugs. 2005; 36 1381-1385
118 Xue C Y, Li D Z, Lu J M, Yang J B, Liu J Q. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. Planta Med. 2006; 72 1223-1226
119 Sahin F P, Yamashita H, Guo Y, Terasaka K, Kondo T, Yamamoto Y, Shimada H, Fujita M, Kawasaki T, Sakai E, Tanaka T, Goda Y, Mizukami H. DNA authentication of Plantago herb based on nucleotide sequences of 18S-28S rRNA internal transcribed spacer region. Biol Pharm Bull. 2007; 30 1265-1270
120 Yang Z Y, Chao Z, Huo K K, Xie H, Tian Z P, Pan S L. ITS sequence analysis used for molecular identification of the Bupleurum species from northwestern China. Phytomedicine. 2007; 14 416-422
121 Xue H G, Zhou S D, He X J, Yu Y. Molecular authentication of the traditional Chinese medicinal plant Euphorbia pekinensis. Planta Med. 2006; 73 91-93
122 Zhao K J, Dong T T, Cui X M, Tu P F, Tsim K W. Genetic distinction of radix adenophorae from its adulterants by the DNA sequence of 5S-rRNA spacer domains. Am J Chin Med. 2003; 31 919-926
123 Carles M, Cheung M K, Moganti S, Dong T T, Tsim K W, Ip N Y, Sucher N J. A DNA microarray for the authentication of toxic traditional Chinese medicinal plants. Planta Med. 2005; 71 580-584
124 Zhao K J, Dong T T, Tu P F, Song Z H, Lo C K, Tsim K W. Molecular genetic and chemical assessment of radix Angelica (Danggui) in China. J Agric Food Chem. 2003; 51 2576-2583
125 Dong T T, Ma X Q, Clarke C, Song Z H, Ji Z N, Lo C K, Tsim K W. Phylogeny of Astragalus in China: molecular evidence from the DNA sequences of 5S rRNA spacer, ITS, and 18S rRNA. J Agric Food Chem. 2003; 51 6709-6714
126 Ma X Q, Duan J A, Zhu D Y, Dong T T, Tsim K W. Species identification of Radix Astragali (Huangqi) by DNA sequence of its 5S-rRNA spacer domain. Phytochemistry. 2000; 54 363-368
127 Xia Q, Zhao K J, Huang Z G, Zhang P, Dong T T, Li S P, Tsim K W. Molecular genetic and chemical assessment of Rhizoma Curcumae in China. J Agric Food Chem. 2005; 53 6019-6026
128 Sun Y, Fung K P, Leung P C, Shi D, Shaw P C. Characterization of medicinal Epimedium species by 5S rRNA gene spacer sequencing. Planta Med. 2004; 70 287-288
129 Cai Z H, Li P, Dong T T, Tsim K W. Molecular diversity of 5S-rRNA spacer domain in Fritillaria species revealed by PCR analysis. Planta Med. 1999; 65 360-364
130 Ma X Q, Zhu D Y, Li S P, Dong T T, Tsim K W. Authentic identification of Stigma Croci (stigma of Crocus sativus) from its adulterants by molecular genetic analysis. Planta Med. 2001; 67 183-186
131 Zhang M, Zhang D Z, Xu X H, Zhang T, Wang Z T. 5S rRNA gene spacer sequences from Ligularia medicinal plants and the identification of HPAs-containing species. Chin J Nat Med. 2005; 3 38-40
132 Sun Y, Shaw P C, Fung K P. Molecular authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii by ITS and 5S rRNA spacer sequencing. Biol Pharm Bull. 2007; 30 173-175
133 Chen F, Chan H Y, Wong K L, Wang J, Yu M T, But P P, Shaw P C. Authentication of Saussurea lappa, an endangered medicinal material, by ITS DNA and 5S rRNA sequencing. Planta Med. 2008; 74 889-892
134 Liu Y P, Cao H, Wang X T. Application of gene technology in quality control of Chinese drugs (II) – Identification of Chinese yam (Dioscorea polystachia rhizome) using DNA sequencing. Chin J Tradit Herb Drugs. 2001; 32 113-117
135 Liu Y P, Cao H, Wang X T. Application of gene technology in quality control of Chinese drugs (I) – identification of Pinellia ternata species from Yuncheng, Shandong using DNA sequencing. Chin J Pharm Anal. 2001; 21 423-426
136 Zhu S, Fushimi H, Cai S, Komatsu K. Phylogenetic relationship in the genus Panax: inferred from chloroplast trnK gene and nuclear 18S rRNA gene sequences. Planta Med. 2003; 69 647-653
137 Wang C Z, Li P, Ding J Y, Jin G Q, Yuan C S. Identification of Fritillaria pallidiflora using diagnostic PCR and PCR-RFLP based on nuclear ribosomal DNA internal transcribed spacer sequences. Planta Med. 2005; 71 384-386
138 Lee S K, Li P T, Lau D T, Yung P P, Kong R Y, Fong W F. Phylogeny of medicinal Phyllanthus species in China based on nuclear ITS and chloroplast atpB-rbcL sequences and multiplex PCR detection assay analysis. Planta Med. 2006; 72 721-726
139 Lee C, Wen J. Phylogeny of Panax using chloroplast trnC-trnD intergenic region and the utility of trnC-trnD in interspecific studies of plants. Mol Phylogenet Evol. 2004; 31 894-903
140 Albach D C, Li H Q, Zhao N, Jensen S R. Molecular systematics and phyotchemistry of Rehmannia (Scrophulariaceae). Biochem Syst Ecol. 2007; 35 293-300
141 Yang M, Zhang D, Liu J, Zheng J. A molecular marker that is specific to medicinal rhubarb based on chloroplast trnL/trnF sequences. Planta Med. 2001; 67 784-786
142 Long C, Kakiuchi N, Takahashi A, Komatsu K, Cai S, Mikage M. Phylogenetic analysis of the DNA sequence of the non-coding region of nuclear ribosomal DNA and chloroplast of Ephedra plants in China. Planta Med. 2004; 70 1080-1084
143 Xue C Y, Li D Z, Lu J M, Yang J B, Liu J Q. Molecular authentication of the traditional Tibetan medicinal plant Swertia mussotii. Planta Med. 2006; 72 1223-1226
144 Hosokawa K, Minami M, Nakamura I, Hishida A, Shibata T. The sequences of the plastid gene rpl16 and the rpl16-rpl14 spacer region allow discrimination among six species of Scutellaria. J Ethnopharmacol. 2005; 99 105-108
145 Hosokawa K, Hishida A, Nakamura I, Shibata T. The sequences of the spacer region between the atpF and atpA genes in the plastid genome allows discrimination among three varieties of medicinal Angelica. Planta Med. 2006; 72 570-571
146 Gong W, Fu C-X, Luo Y-P, Qiu Y-X. Molecular identification of Sinopodophyllum hexandrum and Dysosma species using cpDNA sequences and PCR-RFLP markers. Planta Med. 2006; 72 650-652
147 Zhao Y P, Qiu Y X, Gong W, Li J H, Fu C X. Authentication of Actinidia macrosperma using PCR-RFLP based on trnK sequences. Bot Stud. 2007; 48 239-242
148 Mizukami H, Okabe Y, Kohda H, Hiraoka N. Identification of the crude drug Atractylodes Rhizome (Byaku-jutsu) and Atractylodes lancea Rhizome (So-jutsu) using chloroplast trnK sequence as a molecular marker. Biol Pharm Bull. 2000; 23 589-594
149 Sasaki Y, Fushimi H, Cao H, Cai S-Q, Komatsu K. Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication. Biol Pharm Bull. 2002; 25 1593-1599
150 Luo J P, Cao H, Liu Y P. DNA sequencing and molecular identification of Patchouli and its substitute wrinkled gianthyssop. Yao Xue Xue Bao. 2002; 37 739-742
151 Komatsu K S, Zhu S, Fushimi H, Qui T K, Cai S, Kadota S. Phylogenetic analysis based on 18S rRNA gene and matK gene sequences of Panax vietnamensis and five related species. Planta Med. 2001; 67 461-465
152 Zhao Z L, Leng C H, Wang Z T. Identification of Dryopteris crassirhizoma and the adulterant species based on cpDNA rbcL and translated amino acid sequences. Planta Med. 2007; 73 1230-1233
153 Kondo K, Terabayashi S, Okada M, Yuan C, He S. Phylogenetic relationship of medicinally important Cnidium officinale and Japanese Apiaceae based on rbcL sequences. J Plant Res. 1996; 109 21-27
154 Lin J, Zhou X, Gao S, Wu W, Liu X, Sun X, Tang K. Authentication of Pinellia ternata and its adulterants based on PCR with specific primers. Planta Med. 2006; 72 844-847
155 Maeda M, Uryu N, Murayama N, Ishii H, Ota M, Tsuji K, Inoko H. A simple and rapid method for HLA-DP genotying by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Hum Immunol. 1990; 27 111-121
156 Lum M R, Potter E, Dang T, Heber D, Hardy M, Hirsch A M. Identification of botanicals and potential contaminants through RFLP and sequencing. Planta Med. 2005; 71 841-846
157 Li X, Ding X, Chu B, Ding G, Gu S, Qian L, Wang Y, Zhou Q. Molecular authentication of Alisma orientale by PCR-RFLP and ARMS. Planta Med. 2007; 73 67-70
158 Watanabe A, Araki S, Kobari S, Sudo H, Tsuchida T, Uno T, Kosaka N, Shimomura K, Yamazaki M, Saito K. In vitro propagation, restriction fragment length polymorphism, and random amplified polymorphic DNA analyses of Angelica plants. Plant Cell Rep. 1998; 18 187-192
159 Gong W, Fu C X, Luo Y P, Qiu Y X. Molecular identification of Sinopodophyllum hexandrum and Dysosma species using cpDNA sequences and PCR-RFLP markers. Planta Med. 2006; 72 650-652
160 Guo Y, Tsuruga A, Yamaguchi S, Oba K, Iwai K, Sekita S, Mizukami H. Sequence analysis of chloroplast chlB gene of medicinal Ephedra species and its application to authentication of Ephedra herb. Biol Pharm Bull. 2006; 29 1207-1211
161 Wang C Z, Li P, Ding J Y, Jin G Q, Yuan C S. Identification of Fritillaria pallidiflora using diagnostic PCR and PCR-RFLP based on nuclear ribosomal DNA internal transcribed spacer sequences. Planta Med. 2005; 71 384-386
162 Wang C Z, Li P, Ding J Y, Peng X, Yuan C S. Simultaneous identification of Bulbus Fritillariae cirrhosae using PCR-RFLP analysis. Phytomedicine. 2007; 14 628-632
163 Lee J H, Lee J W, Sung J S, Bang K H, Moon S G. Molecular authentication of 21 Korean Artemisia species (Compositae) by polymerase chain reaction-restriction fragment length polymorphism based on trnL-F region of chloroplast DNA. Biol Pharm Bull. 2009; 32 1912-1916
164 Diao Y, Lin X M, Liao C L, Tang C Z, Chen Z J, Hu Z L. Authentication of Panax ginseng from its adulterants by PCR-RFLP and ARMS. Planta Med. 2009; 75 557-560
165 Do K R, Hwang W J, Lyu Y S, An N H, Kim H M. Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP. Biol Pharm Bull. 2001; 24 872-875
166 Lu K T, Lee H C, Liu F S, Lo C F, Lin J H. Identification of ginseng radix in Chinese medicine preparations by nested PCR-DNA sequencing method and nested PCR-restriction fragment length polymorphism. J Food Drug Anal. 2010; 18 58-63
167 Um J Y, Chung H S, Kim M S, Na H J, Kwon H J, Kim J J, Lee K M, Lee S J, Lim J P, Do K R, Hwang W J, Lyu Y S, An N H, Kim H M. Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP. Biol Pharm Bull. 2001; 24 872-875
168 Zhao Y P, Qiu Y X, Gong W, Li J H, Fu C X. Authentication of Actinidia macrosperma using PCR-RFLP based on trnK sequences. Bot Stud. 2007; 48 239-242
169 Mizukami H, Okabe Y, Kohda H, Hiraoka N. Identification of the crude drug Atractylodes rhizome (Byaku-jutsu) and Atractylodes lancea rhizome (So-jutsu) using chloroplast TrnK sequence as a molecular marker. Biol Pharm Bull. 2000; 23 589-594
170 Mizukami H, Ohbayashi K, Umetsu K, Hiraoka N. Restriction fragment length polymorphism of medicinal plants and crude drugs. II. Analysis of Glehnia littoralis of different geographical origin. Biol Pharm Bull. 1993; 16 611-612
171 Lu K T, Lee H C, Liu F S, Lo C F, Lin J H. Discriminating Astragali Radix from Hedysarum Radix in Chinese Medicine Preparations Using Nested PCR and DNA Sequencing Methods. J Food Drug Anal. 2009; 17 380-385
172 Zhang T, Xu L S, Wang Z T, Zhou K Y, Zhang N, Shi Y F. Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses. Yao Xue Xue Bao. 2005; 40 728-733
173 Mizukami H, Ohbayashi K, Kitamura Y, Ikenaga T. Restriction fragment length polymorphisms (RFLPs) of medicinal plants and crude drugs. I. RFLP probes allow clear identification of Duboisia interspecific hybrid genotypes in both fresh and dried tissues. Biol Pharm Bull. 1993; 16 388-390
174 Fu R Z, Wang J, Zhang Y B, Wang Z T, But P P, Li N, Shaw P C. Differentiation of medicinal Codonopsis species from adulterants by polymerase chain reaction-restriction fragment length polymorphism. Planta Med. 1999; 65 648-650
175 Desmarais E, Lanneluc I, Lagnel J. Direct amplification of length polymorphisms (DALP), or how to get and characterize new genetic markers in many species. Nucleic Acids Res. 1998; 26 1458-1465
176 Ma Y S, Yu H, Li Y Y, Yan H, Cheng X. A study of genetic structure of Stephania yunnanensis (Menispermaceae) by DALP. Biochem Genet. 2008; 46 227-240
177 Ha W Y, Yau F C, But P P, Wang J, Shaw P C. Direct amplification of length polymorphism analysis differentiates Panax ginseng from P. quinquefolius. Planta Med. 2001; 67 587-589
178 Newton C R, Graham A, Heptinstall L E, Powell S J, Summers C, Kalsheker N, Smith J C, Markham A F. Analysis of any point mutation in DNA. The amplification refractory mutation system (ARMS). Nucleic Acids Res. 1989; 17 2503-2516
179 Li X, Ding X, Chu B, Ding G, Gu S, Qian L, Wang Y, Zhou Q. Molecular authentication of Alisma orientale by PCR-RFLP and ARMS. Planta Med. 2007; 73 67-70
180 Zhu S, Fushimi H, Cai S, Komatsu K. Species identification from Ginseng drugs by multiplex amplification refractory mutation system (MARMS). Planta Med. 2004; 70 189-192
181 Diao Y, Lin X M, Liao C L, Tang C Z, Chen Z J, Hu Z L. Authentication of Panax ginseng from its Adulterants by PCR-RFLP and ARMS. Planta Med. 2009; 75 557-560
182 Yang D Y, Fushimi H, Cai S Q, Komatsu K. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and amplification refractory mutation system (ARMS) analyses of medicinally used Rheum species and their application for identification of Rhei Rhizoma. Biol Pharm Bull. 2004; 27 661-669
183 Ding G, Zhang D, Feng Z, Fan W, Ding X, Li X. SNP, ARMS and SSH authentication of medicinal Dendrobium officinale Kimura et Migo and application for identification of Fengdou drugs. Biol Pharm Bull. 2008; 31 553-557
184 Qian L, Ding G, Zhou Q, Feng Z Y, Ding X Y, Gu S, Wang Y, Li X X, Chu B H. Molecular authentication of Dendrobium loddigesii Rolfe by amplification refractory mutation system (ARMS). Planta Med. 2008; 74 470-473
185 Sasaki Y, Fushimi H, Cao H, Cai S Q, Komatsu K. Sequence analysis of Chinese and Japanese Curcuma drugs on the 18S rRNA gene and trnK gene and the application of amplification-refractory mutation system analysis for their authentication. Biol Pharm Bull. 2002; 25 1593-1599
186 Semagn K, Bjørnstad A, Ndjiondjop M N. An overview of molecular marker methods for plants. African J Biotechnol. 2006; 5 2540-2568
187 McDermott J M, Brandle U, Dutly F, Haemmerli U A, Keller S, Muller K E, Wolf M S. Genetic variation in powdery mildew of barley: development of RAPD, SCAR and VNTR markers. Phytopathology. 1994; 4 1316-1321
188 Albani M C, Battey N H, Wilkinson M J. The development of ISSR derived SCAR markers around the saesonal flowering locus (SFL) in Fragaria vesca. Theor Appl Genet. 2004; 109 571-579
189 Paran I, Kesseli R, Michelmore R. Identification of restrictionfragment-length-polymorphism and random amplified polymorphic DNA markers linked to downy mildew resistance genes in lettuce, using near isogenic lines. Genome. 1991; 34 1021-1027
190 Wang J, Ha W Y, Ngan F N, But P P, Shaw P C. Application of sequence characterized amplified region (SCAR) analysis to authenticate Panax species and their adulterants. Planta Med. 2001; 67 781-783
191 Choi Y E, Ahn C H, Kim B B, Yoon E S. Development of species specific AFLP-derived SCAR marker for authentication of Panax japonicus C. A. MEYER. Biol Pharm Bull. 2008; 31 135-138
192 Lee M Y, Doh E J, Park C H, Kim Y H, Kim E S, Ko B S, Oh S E. Development of SCAR marker for discrimination of Artemisia princeps and A. argyi from other Artemisia herbs. Biol Pharm Bull. 2006; 29 629-633
193 Dnyaneshwar W, Preeti C, Kalpana J, Bhushan P. Development and application of RAPD-SCAR marker for identification of Phyllanthus emblica LINN. Biol Pharm Bull. 2006; 29 2313-2316
194 Theerakulpisut P, Kanawapee N, Maensiri D, Bunnag S, Chantaranothai P. Development of species-specific SCAR markers for identification of three medicinal species of Phyllanthus. J Syst Evol. 2008; 46 614-621
195 Devaiah K M, Venkatasubramanian P. Development of SCAR marker for authentication of Pueraria tuberosa (Roxb. ex. Willd.) DC. Curr Sci. 2008; 94 1306-1308
196 Ye Q, Qiu Y X, Quo Y Q, Chen J X, Yang S Z, Zhao M S, Fu C X. Species-specific SCAR markers for authentication of Sinocalycanthus chinensis. J Zhejiang Univ Sci B. 2006; 7 868-872
197 Devaiah K M, Venkatasubramanian P. Genetic characterization and authentication of Embelia ribes using RAPD-PCR and SCAR marker. Planta Med. 2008; 74 194-196
198 Sze S C W, Song J X, Wong R N S, Feng Y B, Ng T B, Tong Y, Zhang K Y B. Application of SCAR (sequence characterized amplified region) analysis to authenticate Lycium barbarum (wolfberry) and its adulterants. Biotechnol Appl Biochem. 2008; 51 15-21
199 Litt M, Luty J A. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am J Hum Genet. 1989; 44 397-401
200 Tautz D. Hypervariablity of simple sequences as a general source of polymorphic DNA markers. Nucleic Acids Res. 1989; 17 6463-6471
201 Gupta P K, Balyan H S, Sharma P C, Ramesh B. Microsatellites in plants: a new class of molecular markers. Curr Sci. 1996; 70 45-54
202 Kim J, Jo B H, Lee K L, Yoon E S, Ryu G H, Chung K W. Identification of new microsatellite markers in Panax ginseng. Mol Cells. 2007; 24 60-68
203 Jo B H, Suh D S, Cho E M, Kim J, Ryu G H, Chung K W. Characterization of polymorphic microsatellite loci in cultivated and wild Panax ginseng. Genes Genomics. 2009; 31 119-127
204 Kim J, Chung K W. Isolation of new microsatellite-containing sequences in Acanthopanax senticosus. J Plant Biol. 2007; 50 557-561
205 Fan W J, Luo Y M, Li X X, Gu S, Xie M L, He J, Cai W T, Ding X Y. Development of microsatellite markers in Dendrobium fimbriatum Hook, an endangered Chinese endemic herb. Mol Ecol Res. 2009; 9 373-375
206 Kumar J, Verma V, Shahi A K, Qazi G N, Balyan H S. Development of simple sequence repeat markers in Cymbopogon species. Planta Med. 2007; 73 262-266
207 Chun S, Jian-He W, Shi-Lin C, Huai-Qiong C, Cheng-Min Y. Development of genomic SSR and potential EST-SSR markers in Bupleurum chinense DC. African J Biotechnol. 2009; 8 6233-6240
208 Boqian Y, Jing W, Guopei C, Ting W. Isolation and characterization of polymorphic microsatellite loci in a traditional Chinese medicinal plant, Schisandra sphenanthera. Conserv Genet. 2009; 10 615-617
209 Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. AFLE a new technique for DNA fingerprinting. Nucleic Acids Res. 1995; 23 4407-4441
210 Paglia G E, Olivieri A M, Morgante M. Towards second-generation STS (sequence-tagged sites) linkage maps in conifers, a genetic map of Norway spruce (Picea abies K.). Mol Gen Genet. 1998; 258 466-478
211 Karp A, Edwards K J. DNA markers, a global overview. Caetano-Anollds G, Gresshoff PM DNA Markers, Protocols, Applications, and Overviews. New York; Wiley-Liss 1997
212 Molina C, Kahl G. Genomics of two banana pathogens, genetic diversity, diagnostics, and phylogeny of Mycosphaerella fijiensis and M. musicola. Jain SM Banana Improvement, Cellular and Molecular Biology, and Induced Mutations. Vienna; FAO/IAEA 2002
213 Winter E, Pfaff T, Udupa S M, Hiittel B, Sharma P C, Sahi S, Arreguin-Espinoza R, Weigand F, Muehlbauer F J, Kah G. Characterization and mapping of sequence-tagged microsatellite sites in the chickpea (Cicer arietinum L.) genome. Mol Gen Genet. 1999; 262 90-101
214 Witsenboer H, Vogel J, Michelmore R W. Identification, genetic localization, and allelic diversity of selectively amplified polymorphic loci in lettuce and wild relatives (Lactuca spp.). Genome. 1997; 40 923-936
215 Sarwat M, Das S, Srivastava P S. Analysis of genetic diversity through AFLP, SAMPL, ISSR and RAPD markers in Tribulus terrestris, a medicinal herb. Plant Cell Rep. 2008; 27 519-528
216 Heath D D, Iwama G K, Devlin R H. PCR primed with VNTR core sequences yields species specific patterns and hypervariable probes. Nucleic Acids Res. 1993; 21 5782-5785
217 Somers D J, Demmon G. Identification of repetitive, genome-specific probes in crucifer oilseed species. Genome. 2002; 45 485-492
218 Silva L M, Montes de Oca H, Diniz C R, Fortes-Dias C L. Fingerprinting of cell lines by directed amplification of minisatellite-region DNA (DAMD). Braz J Med Biol Res. 2001; 34 1405-1410
219 Zhou Z, Bebeli P J, Somers D J, Gustafson J P. Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza. Theor Appl Genet. 1997; 95 942-949
220 Ha W Y, Shaw P C, Liu J, Yau F, Wang J. Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD). J Agric Food Chem. 2002; 50 1871-1875
221 Ince A G, Karaca M, Onus A N. Development and utilization of diagnostic DAMD-PCR markers for Capsicum accessions. Genet Resour Crop Evol. 2009; 56 211-221
222 Karaca M, Ince A G, Tugrul S, Turgut K, Onus A N. PCR-RFLP and DAMD-PCR genotyping for Salvia species. J Sci Food Agric. 2008; 88 2508-2516
223 Bhattacharya E, Dandin S B, Ranade S A. Single primer amplification reaction methods reveal exotic and indigenous mulberry varieties are similarly diverse. J Biosci. 2005; 30 669-677
224 Chavan P, Joshi K, Patwardhan B. DNA microarrays in herbal drug research. Evid Based Complement Alternat Med. 2006; 3 447-457
225 Gebauer M. Microarray applications: emerging technologies and perspectives. Drug Discov Today. 2004; 9 915-917
226 Debouck C, Goodfellow P N. DNA microarrays in drug discovery and development. Nat Genet. 1999; 21 48-50
227 Trau D, Lee T M, Lao A I, Lenigk R, Hsing I M, Ip N Y. Genotyping on a complementary metal oxide semiconductor silicon polymerase chain reaction chip with integrated DNA microarray. Anal Chem. 2002; 74 3168-3173
228 Tsoi P Y, Wu H S, Wong M S, Chen S L, Fong W F, Xiao P G, Yang M S. Genotyping and species identification of Fritillaria by DNA chip technology. Acta Pharm Sin. 2003; 4 185-190
229 Li T, Wang J, Lu Z. Accurate identification of closely related Dendrobium species with multiple species-specific gDNA probes. J Biochem Biophys Methods. 2005; 62 111-123
230 Zhang Y B, Wang J, Wang Z T, But P P, Shaw P C. DNA microarray for identification of the herb of Dendrobium species from Chinese medicinal formulations. Planta Med. 2003; 69 1172-1174
231 Lin W Y, Chen L R, Lin T Y. Rapid authentication of Bupleurum species using an array of immobilized sequence-specific oligonucleotide probes. Planta Med. 2008; 74 464-469
232 Qin J, Leung F C, Fung Y, Zhu D, Lin B. Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection. Anal Bioanal Chem. 2005; 381 812-819
233 Carles M, Cheung M K, Moganti S, Dong T T, Tsim K W, Ip N Y, Sucher N J. A DNA microarray for the authentication of toxic traditional Chinese medicinal plants. Planta Med. 2005; 71 580-584
234 Pastinen T, Partanen J, Syvanen A C. Multiplex, fluorescent, solid-phase minisequencing for efficient screening of DNA sequence variation. Clin Chem. 1996; 42 1391-1397
235 Cai H, White P S, Torney D, Deshpande A, Wang Z, Keller R A, Marrone B, Nolan J P. Flow cytometry-based minisequencing: a new platform for high-throughput single-nucleotide polymorphism scoring. Genomics. 2000; 66 135-143
236 Pastinen T, Raitio M, Lindroos K, Tainola P, Peltonen L, Syvänen A C. A system for specific, high-throughput genotyping by allele-specific primer extension on microarrays. Genome Res. 2000; 10 1031-1042
237 Lowe C R. Nanobiotechnology: the fabrication and applications of chemical and biological nanostructures. Curr Opin Struct Biol. 2000; 10 428-434
238 Fortina P, Kricka L J, Surrey S, Grodzinski P. Nanobiotechnology: the promise and reality of new approaches to molecular recognition. Trends Biotechnol. 2005; 23 168-173
239 Pirrung M C, Connors R V, Odenbaugh A L, Montague-Smith M P, Walcott N G, Tollett J J. The arrayed primer extension method for DNA microchip analysis. Molecular computation of satisfaction problems. J Am Chem Soc. 2000; 122 1873-1882
240 Kurg A, Tõnisson N, Georgiou I, Shumaker J, Tollett J, Metspalu A. Arrayed primer extension: solid-phase four-color DNA resequencing and mutation detection technology. Genet Test. 2000; 4 1-7
241 Grossman P D, Bloch W, Brinson E, Chang C C, Eggerding F A, Fung S, Iovannisci D M, Woo S, Winn-Deen E S, Iovannisci D A. High-density multiplex detection of nucleic acid sequences: oligonucleotide ligation assay and sequence-coded separation. Nucleic Acids Res. 1994; 22 4527-4534
242 Cimino M T. Successful isolation and PCR amplification of DNA from National Institute of Standards and Technology herbal dietary supplement standard reference material powders and extracts. Planta Med. 2010; 76 495-497

Prof. Dr. Günther Heubl

Department Biologie I – Systematische Botanik
LMU München

Menzingerst. 67

80638 München

Germany

Telefon: +49 89 17 86 12 07

Fax: +49 89 17 26 38

eMail: heubl@lrz.uni-muenchen.de