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DOI: 10.1055/s-0030-1250519
© Georg Thieme Verlag KG Stuttgart · New York
New Aspects of DNA-based Authentication of Chinese Medicinal Plants by Molecular Biological Techniques
Publication History
received May 12, 2010
revised October 4, 2010
accepted October 7, 2010
Publication Date:
05 November 2010 (online)
![](https://www.thieme-connect.de/media/plantamedica/201017/lookinside/thumbnails/10.1055-s-0030-1250519-1.jpg)
Abstract
DNA technology provides a powerful tool to complement chemical analyses for authentication of Chinese medicinal plants and to ensure that herbal materials are not contaminated with ineffective or potentially harmful substitutes or adulterants. In the last two decades molecular biotechnology has provided sophisticated molecular techniques for authentication of botanical materials at the DNA level. This review provides an account of the most commonly used DNA-based technologies (RAPD, RFLP, ARMS, CAPS, AFLP, DAF, ISSR, SSR, sequencing, hybridization and microarrays) including suitable examples of Chinese medical plants. A critical evaluation of all methods is presented concerning sensitivity, reliability, reproducibility, and running costs. Recent achievements in the field of DNA barcoding and DNA chip technology that offer great potentials for screening of DNA and emerging new developments for future identification of species are briefly outlined.
Key words
authentication - species identification - Chinese medicinal plants - standardization - molecular markers - DNA technologies
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Prof. Dr. Günther Heubl
Department Biologie I – Systematische Botanik
LMU München
Menzingerst. 67
80638 München
Germany
Phone: +49 89 17 86 12 07
Fax: +49 89 17 26 38
Email: heubl@lrz.uni-muenchen.de