Planta Med 2010; 76 - P94
DOI: 10.1055/s-0030-1251856

Evaluation of Antiulcerogenic Activity of Tephrosia calophylla Roots on Aspirin Plus Pyloric Ligation-induced Ulcers in Experimental Rats

K Adinarayana 1, KN Jayveera 2, MR Pichika 3
  • 1Annamacharya College of Pharmacy, Rajampet, 516115, Kadapa Dist., Andhra Pradesh state, India
  • 2Chemical & Pharmaceutical Sciences and Oil Technological Research Institute, Jawaharlal Nehru Technological University, Anantapur, 515001, Andhra Pradesh state, India
  • 3School of Pharmacy and Health Sciences, International Medical University, No. 126, Jalan 19/55B, Bukit Jalil 57000 Kuala Lumpur, Malaysia

Tephrosia calophylla Bedd (Fabaceae) roots are useful in the treatment of gastric disorders in Indian traditional system of medicine. The present study describes antiulcerogenic potential of T. calophylla roots on aspirin plus pyloric ligation induced gastric ulcer model to substantiate its folklore claim. Its mechanism of action is elucidated by evaluating its secretory action, mucin formation, involvement of NO, SH, PGE2, PCNA, COX-2 and anti-Helicobacter pylori activity. The antiulcerogenic potential of ethylacetate extract of the root powder (ETCR) at doses of 50, 100, 150, 200 and 250mg/kg, p.o. prevented ulcer formation without antisecretory effect. The antiulcerogenic potential was further confirmed by the histopathological studies of stomach mucosa. ETCR showed anti-Helicobacter pylori action (MIC=15ng/mL) and was able to inhibit NO production by macrophages. ETCR also accelerate the healing of ulcerated gastric mucosa by stimulating the proliferation factors (PCNA), COX-2 and maintained basal PGE2 level. Three coumestan derivatives were isolated from ETCR. These compounds may be responsible for antiulcerogenic activity of T. calophylla roots.